Mouse fear conditioning chamber

Manufactured by Med Associates

The Mouse Fear Conditioning Chamber is a laboratory equipment designed for the study of fear and learning in mice. The apparatus consists of a small enclosed chamber with a floor that can deliver mild electrical shocks. It is used to measure and record the behavioral responses of mice during fear conditioning experiments.

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Conditioning chambers (60 citations) Fear conditioning chambers (47 citations)

4 protocols using mouse fear conditioning chamber

Fear Conditioning and Startle Threshold

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Fear conditioning was performed as described previously (Kim et al., 2014 (link); Porton et al., 2010 (link)). Mouse fear conditioning chamber (Med Associates, Inc) was used for conditioning and testing. Following a 2 min acclimation in the conditioning chamber, mice received a 0.4 mA scrambled foot shock for 2 s. Each mouse remained in the chamber for an additional 30 s before being placed in the home cage. Fear contextual memory was tested the next day in the same conditioning chamber for 5 min in the absence of the foot shock. Startle shock threshold was conducted with the Med-Associates startle platform (St Albans, VT) run by Startle Pro Software. Mice were placed onto a multibar grid in a Plexiglass tube which allowed the animal to move freely back and forth, but not rears upright. After 2 min of acclimation, the mouse was subjected to a total of 10 scrambles of intensities from 0 to 0.6mA separated by varying intervals of 20–90 s. Startle responses by the animal during the first 100 msec of the shock were transduced through the load cells in the holding platform. The area under the curve (AUC) of the startle reactivity was measured and expressed as arbitrary units (AU).

Uezu A., Hisey E., Kobayashi Y., Gao Y., Bradshaw T.W., Devlin P., Rodriguiz R., Tata P.R, & Soderling S. (2019). Essential role for InSyn1 in dystroglycan complex integrity and cognitive behaviors in mice. eLife, 8, e50712.

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Delayed Fear Conditioning Assay

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A delayed fear conditioning protocol was employed to evaluate hippocampus-dependent contextual fear memory and hippocampus-independent cue fear memory. On day 0 animals were trained in a mouse fear conditioning chamber with a grid floor that can deliver an electric shock (Med Associates, Inc.). This enclosure was located in a sound-attenuating box that contained a digital camera, a loudspeaker and a house light. Each mouse was initially placed in the chamber and left undisturbed for 2 min, after which a tone (30 s, 5 kHz, 80 dB) coincided with a scrambled foot shock (2 s, 0.7 mA). The tone/foot-shock stimuli were repeated after 2 min. The mouse was then returned to its home cage. The context test was assessed in 24 hours. The mice were placed in exactly the same environment and observed for 5 min. The cued fear test was assessed one hour after the context test. The mice were placed in a novel environment for 3 min, followed by a 3 min tone. Mouse behaviour was recorded and scored automatically by ANY-maze (Stoelting). Freezing, defined as the absence of all movement except for respiration, was scored only if the animal was immobile for at least 1 s. The percentage of time spent freezing during the tests serves as an index of fear memory.

Shao Y., Sztainberg Y., Wang Q., Bajikar S.S., Trostle A.J., Wan Y.W., Jafar-nejad P., Rigo F., Liu Z., Tang J, & Zoghbi H.Y. (2021). Antisense oligonucleotide therapy in a humanized mouse model of MECP2 duplication syndrome. Science translational medicine, 13(583), eaaz7785.

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Fear Conditioning in Mice: A Standardized Approach

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Fear conditioning testing was conducted in mouse fear‐conditioning chambers (Med Associates) as described.¹⁹, ²² Mice were tested using a 3‐day paradigm with conditioning on day 1, context testing 24 h later and cued testing on the third day. Conditioning consisted placing the mouse in the test apparatus for 2 min after which a 72 dB 12 kHz tone (conditioned stimulus or CS) was presented for 30 s and was terminated with a 2 s 0.4 mA scrambled foot‐shock (unconditioned stimulus or UCS). After 30 s the animal was returned to its home‐cage. For context testing the mouse was exposed to the conditioning chamber for 5 min in the absence of the CS and UCS. Cued testing was conducted in a novel chamber that was different from the conditioning chamber in illumination, dimensions, shape and with a different floor and walls. Mice were placed into the chamber for 2 min, after which the CS was presented for 3 min. All responses were videotaped and scored later with the Noldus Observer program for freezing behaviors by trained observers who were blinded to the genotypes of the mice. Freezing was defined by the absence of all visible movement except that required for respiration.

Aryal D.K., Rodriguiz R.M., Nguyen N.L., Pease M.W., Morgan D.J., Pintar J., Fricker L.D, & Wetsel W.C. (2022). Mice lacking proSAAS display alterations in emotion, consummatory behavior and circadian entrainment. Genes, Brain, and Behavior, 21(7), e12827.

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Fear Conditioning Behavioral Assay in Mice

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Fear conditioning testing was conducted in mouse fear-conditioning chambers (Med Associates) as described.^{19 ,22 (link)} Mice were tested using a 3-day paradigm with conditioning on day 1, context testing 24 hr later, and cued testing on the third day. Conditioning consisted placing the mouse in the test apparatus for 2 min after which a 72 dB 12 kHz tone (conditioned stimulus or CS) was presented for 30 sec and was terminated with a 2-sec 0.4 mAmp scrambled foot-shock (unconditioned stimulus or UCS). After 30 sec the animal was returned to its home-cage. For context testing the mouse was exposed to the conditioning chamber for 5 min in the absence of the CS and UCS. Cued testing was conducted in a novel chamber that was different from the conditioning chamber in illumination, dimensions, shape, and with a different floor and walls. Mice were introduced into the chamber for 2 min, after which the CS was presented for 3 min. All responses were videotaped and scored later with the Noldus Observer program for freezing behaviors by trained observers who were blinded to the genotypes of the mice. Freezing was defined by the absence of all visible movement except that required for respiration.

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