Dulbecco’s modified Eagle’s medium (DMEM), RPMI 1640 medium, fetal bovine serum, penicillin/streptomycin (10,000 units/mL penicillin and 10,000 µg/mL streptomycin), and 0.25% trypsin-EDTA were purchased from Gibco, Thermo Fisher Scientific, Inc. (USA). JC-1 dye was obtained from Invitrogen, Thermo Fisher Scientific, Inc. (USA). Nar, APAP, dorsomorphin (Dor), and brusatol (Bru) were obtained from MedChemExpress (USA) and dissolved in dimethyl sulfoxide (DMSO). A malondialdehyde (MDA) assay kit (TBA method), superoxide dismutase (SOD) assay kit (WST-1 method), reduced GSH assay kit, and glutamate dehydrogenase (GDH) test kit were obtained from Nanjing Jiancheng Bioengineering Institute (China). A reactive oxygen species (ROS) assay kit was obtained from Beyotime (China). A CCK8 Cell Proliferation and Cytotoxicity Assay Kit was obtained from Solarbio (China).
Antibodies specific for β-actin were purchased from Proteintech Group, Inc. (China). Antibodies specific for mitofusin protein 1 (Mfn1), optic atrophy type 1 (Opa1), phospho (p)-dynamin-related protein 1 (Drp1) (Ser616), total Drp1, AMPK, p-AMPK (Thr172), Nrf2, and GAPDH were purchased from Cell Signaling Technology (USA).
Antibodies specific for β-actin were purchased from Proteintech Group, Inc. (China). Antibodies specific for mitofusin protein 1 (Mfn1), optic atrophy type 1 (Opa1), phospho (p)-dynamin-related protein 1 (Drp1) (Ser616), total Drp1, AMPK, p-AMPK (Thr172), Nrf2, and GAPDH were purchased from Cell Signaling Technology (USA).