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Human elisa assays

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Human ELISA assays are a type of immunoassay used to detect and quantify specific proteins or other analytes in human biological samples. The assay utilizes antibodies and color changes to measure the concentration of the target analyte in the sample.

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Lab products found in correlation

Bca protein assay, by Thermo Fisher Scientific (2 mentions) Ripa buffer, by Merck Group (1 mentions) Protease phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)

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ELISAs (35 citations) ELISA assays (15 citations) Human MMP-9 ELISA assays (3 citations)

2 protocols using human elisa assays

1

Acetone Precipitation for Protein Concentration

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Media protein was concentrated using ice-cold 100% acetone at a 2:1 ratio. Media samples were incubated in acetone for a minimum of 30 min at −20°C. Samples were sedimented by centrifugation at 5,000 × g for 5 min followed by a 70% ethanol wash. Samples were re-suspended in 8 M urea. Media samples were used in downstream ELISA assays. Aβ40 and Aβ42 were measured using human ELISA assays from ThermoFisher with samples diluted 1:5. All data were normalized to the protein content of the samples using a BCA protein assay (ThermoFisher).
Flannagan K., Stopperan J.A., Hauger B.M., Troutwine B.R., Lysaker C.R., Strope T.A., Csikos Drummond V., Gilmore C.A., Swerdlow N.A., Draper J.M., Gouvion C.M., Vivian J.L., Haeri M., Swerdlow R.H, & Wilkins H.M. (2023). Cell type and sex specific mitochondrial phenotypes in iPSC derived models of Alzheimer’s disease. Frontiers in Molecular Neuroscience, 16, 1201015.
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2

Acetone Precipitation and ELISA Quantification

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Media protein was concentrated using ice-cold 100% acetone at a 2:1 ratio. Media samples were incubated in acetone for a minimum of 30 minutes at −20°C. Samples were sedimented by centrifugation at 5,000 × g for 5 minutes followed by a 70% ethanol wash. Samples were resuspendend in 8M urea. Whole cell lysate samples were prepared in RIPA buffer (Sigma Aldrich) with protease/phosphatase inhibitor cocktail (ThermoFisher). Media and whole cell samples were used in downstream ELISA assays. Aβ40 and Aβ42 were measured using human ELISA assays from ThermoFisher with samples at a dilution of 1:5. All data were normalized to the protein content of the samples using a BCA protein assay (ThermoFisher).
Wilkins H.M., Troutwine B.R., Menta B.W., Manley S.J., Strope T.A., Lysaker C.R, & Swerdlow R.H. (2022). Mitochondrial Membrane Potential Influences Amyloid Precursor Protein Localization and Aβ Secretion. Journal of Alzheimer's disease : JAD, 85(1), 381-394.
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