Hinokitiol (469521) was purchased from Sigma (St. Louis, MO, USA). Stock solutions of 10 mM Hinokitiol was dissolved in dimethyl sulfoxide (DMSO, Sigma-Aldrich) and stored at -80°C. Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies Inc. (Kumamoto, Japan). Annexin V-FITC/propidium iodide (PI) apoptosis straining kit was purchased from BD company (American). The mRFP-GFP-LC3 adenovirus construct was obtained from Hanbio Inc. (Shanghai, China). Hochest33258 was purchased from Kaiji Biology (Jiangsu, China). All the antibodies used were as follows: P62 (#88588), Beclin-1 (#3495), caspase-3 (#9664), Bcl-2 (#4223), p-mTOR (#D9C2), and p-GSK3β (Ser9) (#9336) were obtained from Cell Signaling Technology (Beverly, USA). P21 (#55643) was purchased from BD PharMingen (New York, USA). GAPDH (60004) was purchased from Proteintech Group, Inc. (USA).
P mtor d9c2
Manufactured by Cell Signaling Technology
Sourced in United States
P-mTOR (#D9C2) is a primary antibody that detects phosphorylated mTOR (Ser2448). mTOR is a serine/threonine protein kinase that acts as a master regulator of cell growth and metabolism.
Automatically generated - may contain errors
Lab products found in correlation
Hinokitiol, by Merck Group (1 mentions)
Bcl2 4223, by Cell Signaling Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
P gsk3β ser9, by Cell Signaling Technology (1 mentions)
C ebpβ, by Santa Cruz Biotechnology (1 mentions)
Mtor 7c10, by Cell Signaling Technology (1 mentions)
Antibody to actin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using p mtor d9c2
1
Hinokitiol-Induced Autophagy and Apoptosis
2
Protein Expression Analysis in Cell Lysates
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Cells were lysed by using a sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) sample loading buffer. The lysates were subjected to PAGE and transferred onto a nitrocellulose membrane. The membranes were blocked with 5% nonfat dried milk and probed with the following specific primary antibodies: C/EBP‐β, sterol regulatory element binding protein (SREBP)‐1 (2A4), and fatty acid synthase (FASN) (A‐5; Santa Cruz); ATG5 (Proteintech, IL); and mammalian target of rapamycin (mTOR; 7C10) and phosphorylated‐mTOR (p‐mTOR; D9C2) (Cell Signaling Technology, Danvers, MA). After washing, the blots were incubated with secondary antibody for 1 hour. Proteins were detected by using an enhanced chemiluminescence western blot substrate (Pierce; ThermoFisher Scientific). Membranes were reprobed with antibody to actin (Santa Cruz) as an internal control for normalization of the protein load. ImageJ software (National Institutes of Health [NIH]) was used for densitometric scanning of western blot images.
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