C57BL/6J (B6) male mice, female non-obese diabetic (NOD/ShiLtJ) mice, female non-obese diabetes-resistant (NOR/LtJ), and INS1cre (B6(Cg)-Ins1tm1.1(cre)Thor/J) were obtained from the Jackson Laboratories. TMEM219flox/flox were generated in collaboration with Applied StemCell (Milpitas, CA) and housed at Charles River Laboratories (Wilmington, MA). TMEM219flox/floxINS1cre male mice (Beta-TMEM219−/−) were obtained by breeding TMEM219flox/flox mice with INS1cre mice, and the resulting colony was housed at Charles River Laboratories. Mice were housed at a temperature between 21 and 23 °C with 50–60% humidity and kept on a 12/12 h light/dark cycle with free access to food and water. All mice were cared for and used in accordance with institutional guidelines approved by the Boston Children’s Hospital Institutional Animal Care and Use Committee or in accordance with Italian law on animal care N° 116/1992 and the European Communities Council Directive EEC/609/86. All animal studies were approved by the Italian Ministry of Health and Local University of Milan Committee and by the Boston Children’s Hospital Institutional Animal Care and Use Committee. Some studies were conducted at the Jackson Laboratories.
Nor ltj
Manufactured by Jackson ImmunoResearch
Sourced in United States
The NOR/LtJ is a laboratory product offered by Jackson ImmunoResearch. It serves as a core functional component for specific research applications. The details of its intended use and application are not included in this factual description.
Automatically generated - may contain errors
Lab products found in correlation
Nod shiltj, by Jackson ImmunoResearch (2 mentions)
C57bl 6, by Jackson ImmunoResearch (2 mentions)
C57bl 6j b6 male mice, by Jackson ImmunoResearch (1 mentions)
Nod shiltj mice, by Jackson ImmunoResearch (1 mentions)
Nod cb17 prkdcscid j nod scid, by Jackson ImmunoResearch (1 mentions)
Female nod shiltj, by Jackson ImmunoResearch (1 mentions)
C57bl 6j b6, by Jackson ImmunoResearch (1 mentions)
Nod mice, by Taconic Biosciences (1 mentions)
B10 br mice, by Jackson ImmunoResearch (1 mentions)
Complete protease inhibitor, by Roche (1 mentions)
C3h hej, by Jackson ImmunoResearch (1 mentions)
129s1 svimj, by Jackson ImmunoResearch (1 mentions)
Fvb nj, by Jackson ImmunoResearch (1 mentions)
Balb cj, by Jackson ImmunoResearch (1 mentions)
7 protocols using nor ltj
1
Generation and Analysis of Transgenic Mice
2
Murine Models for Immunological Studies
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Female NOD/ShiLtJ (NOD), NOD.CB17-Prkdcscid/J (NOD.Scid), NOR/LtJ, NOD.BDC2.5 (BDC2.5), and C57BL/6J (B6) mice were purchased from The Jackson Laboratory (Bar Harbor, ME). Human CD20 transgenic NOD mice [hCD20(+/+) NOD] were generated and maintained as previously reported (15 (link)). All mice were used according to institutional guidelines, and animal protocols were approved by the Boston Children’s Hospital Institutional Animal Care and Use Committee.
3
Lacrimal Gland Tissue Extraction and Analysis
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All experiments described herein were performed in accordance with the Association for Research in Vision and Ophthalmology (ARVO) Statement for the Use of Animals in Ophthalmic and Vision Research and were approved by the Tufts Medical Center Animal Care and Use Committee, the University of Southern California Institutional Animal Care and Use Committee, the Scripps Research Institute Animal Care and Use Committee, and Regierung von Unterfranken (Friedrich Alexander University Erlangen-Nürnberg). MRL/MpJ-Fas /J (MRL/lpr; female, 12 weeks old) and NOR/LtJ (NOD; male, 13 weeks old) mice and their respective age- and sex-matched MRL/MpJ (MRL +/+) and BALB/c control mice were purchased from the Jackson Laboratories (Bar Harbor, ME). We also used in-house bred, 12–13-week-old male NOD mice that were purchased from Taconic Biosciences (Hudson, NY). Animals were euthanized and the exorbital lacrimal glands were harvested and processed for paraffin embedding, total protein extraction, RNA extraction or stimulation with oxytocin. In other experiments, SMA-GFP mice (kind gift of Dr. Ivo Kalajzic), in which lacrimal gland MECs are labeled with GFP, were used to isolate MECs from the lacrimal gland by fluorescence-activated cell sorting (FACS).
4
Islet Isolation from NOD/ShiLtJ and NOR/LtJ Mice
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Female 6-week-old non-obese diabetic NOD/ShiLtJ (#001976) and non-obese diabetes-resistant NOR/LtJ (#002050) mice were purchased from Jackson Labs. The husbandry of mice and experimental procedures were performed according to an approved IACUC protocol at the University of Colorado (#00045). Mice were housed for < 1 week prior to islet isolation. Approximately 320–520 islets (n = 3) were isolated using a Histopaque gradient centrifugation and further handpicking of islets as described previously [17 (link)].
5
Glucose Monitoring in Diabetic Mouse Models
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Female NOD/ShiLt, NOR/LtJ, C57BL/6, and B10.BR mice were purchased at three weeks of age from Jackson Laboratories (Bar Harbor, Maine). All animal studies were performed in accordance with the guidelines of the Yale University Institutional Animal Care and Use Committee. All mice were housed in microisolator cages with free access to food and water and maintained under the following facility conditions: temperature: 72 °C ± 2 °C; humidity: 50% ± 30%; 12:12 light/dark cycle (i.e., 12 h of light from 7 a.m.–7 p.m. then 12 h of dark from 7 p.m.–7 a.m.). Glucose levels were measured in blood withdrawn from the retroorbital sinus of anesthetized mice using Lite glucometer and test strips (Abbott Laboratories, Abbott Park, IL, USA). Mice with blood glucose values greater than 250 mg/dL were considered diabetic. Serial serum samples were acquired from NOD and age matched control animals. Pancreas and liver tissue extract were prepared in RIPA buffer containing a mixture of protease inhibitors (Complete protease inhibitor, Roche Diagnostics).
6
Murine Model for Diabetes Research
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Male NOR (non-obese diabetic resistant) mice were obtained from The Jackson Laboratory (Bar Harbor, ME, USA) (NOR/LtJ, stock #002050) and housed under specific pathogen-free conditions in our Animal Facility. Mice were kept under controlled temperature (21 °C), humidity (55–60%) and light conditions (12 h light:12 h darkness cycle) and with ad libitum access to food and water. All experimental procedures involving animals were conducted in accordance with the European Union Directive 2010/63/EU and approved by the national competent authority (Authorization No. 590/13.01.2021).
7
Murine Immunological Strain Characterization
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All mice were maintained in specific pathogen free animal facilities either at the Maisonneuve-Rosemont Hospital Research Center or at the University of Alberta Health Sciences and Laboratory Animal Services. NOD/SHiLtJ (NOD, #001976), C57BL/6 (B6, #000664), B6.SJL-Ptprc a Pepc b /BoyJ (B6.SJL, #002014), B6.NOD-(D17Mit21-D17Mit10)/LtJ (B6 g7 , #003300), NOR/LtJ (#002050), 129S1/SvImJ (#002448), A/J (#000646), BALB/cJ (#000651), FVB/NJ (#001800), and C3H/HeJ (#000659) were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). B6 x B6SJL (CD45.1.2 B6) and NOD x B6 g7 F1 (F1 g7 ) were bred in house. B6.Rag2pGFP (B6.Rag-GFP) mice 63, 64 were kindly provided by Pamela Fink (University of Washington, Seattle, WA, USA) and bred in house and were crossed to NOD mice for more than 14 generations to generate NOD.Rag-GFP mice. Both male and female mice aged 6 -9 weeks were used except as noted. All protocols have been approved by the Animal Care Committee at Maisonneuve-Rosemont Hospital Research Centre and the Animal Care and Use Committee Health Sciences of the University of Alberta. Experiments were performed in accordance with the Canadian Council on Animal Care guidelines.
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