All reagents were analytical grade. 1,8-Octanediol, calcium pantothenate, citric acid, chloroform, clofazimine (CFZ), coumarin 6 (Cou-6), dimethyl 2-oxoglutarate, dimethyl sulfoxide (DMSO) anhydrous, diphenyl ether, Dulbecco’s Modified Eagle’s Medium - high glucose (DMEM), hexane, L-leucine, poly(vinyl alcohol) (PVA; Mw 31,000–50,000; 98–99% hydrolysed), phosphate buffered saline tablets, phenol red, phorbol 12-myristate 13-acetate (PMA), silicon oil, sodium phosphate dibasic, Tween 80 and tricaine were purchased from Sigma-Aldrich, Merck (UK). Dichloromethane, formaldehyde, hygromycin B, methanol, Middlebrook 7H9 with OADC, Remel Middlebrook 7H10 Agar (Dehydrated) and trifluoroacetic acid (TFA) were acquired from Thermo Fisher Scientific (UK). Dimethyl sulphoxide-[D₆] (99.8% D), fetal calf serum (FCS; Sera Plus, EU approved regions, special processed FBS, 0.2 μm sterile filtered) and macrophage colony stimulating factor (MCSF) were purchased from VWR (UK), PanBiotech (Germany) and Peprotech (UK), respectively.
Calcium pantothenate
Manufactured by Merck Group
Sourced in United Kingdom, United States
Calcium pantothenate is a chemical compound that is the calcium salt of pantothenic acid, also known as vitamin B5. It is a water-soluble vitamin that plays a crucial role in various metabolic processes within the body. As a lab equipment product, calcium pantothenate is used as a nutrient supplement or as a component in cell culture media for cell growth and maintenance.
Automatically generated - may contain errors
Lab products found in correlation
Tween 80, by Merck Group (4 mentions)
Nicotinic acid, by Merck Group (4 mentions)
L leucine, by Merck Group (3 mentions)
Biotin, by Merck Group (3 mentions)
Albumin, by Merck Group (2 mentions)
Dextrose, by Merck Group (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Riboflavin, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dichloromethane, by Thermo Fisher Scientific (1 mentions)
Silicon oil, by Merck Group (1 mentions)
Formaldehyde, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline tablet, by Merck Group (1 mentions)
Tricaine, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Thermo Fisher Scientific (1 mentions)
1 8 octanediol, by Merck Group (1 mentions)
Phenol red, by Merck Group (1 mentions)
Clofazimine, by Merck Group (1 mentions)
Anhydrous dimethyl sulfoxide dmso, by Merck Group (1 mentions)
Middlebrook 7h9 with oadc, by Thermo Fisher Scientific (1 mentions)
10 protocols using calcium pantothenate
1
Formulation and Characterization of Clofazimine Nanoparticles
2
Isolation and Cultivation of Lactobacillus helveticus
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Lactobacillus helveticus CICC22171 were isolated from traditional Chinese cheeses in the microbiology laboratory of Beijing Forestry University. The strain was deposited at the China Industrial Culture Collection (CICC). Glucose, 20 basic amino acids, vitamins (nicotinic acid, calcium pantothenate, pyridoxal, riboflavin, biotin, folic acid, and thiamine), sodium acetate, MgSO4, K2HPO4, MnSO4, Tween 80, and casein acid hydrolysate were purchased from Sigma-Aldrich Corp. (St. Louis, MO, United States).
3
Fluorescent Mycobacterium Strains for Live Imaging
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M. marinum M strain (ATCC #BAA-535) and its mutant derivatives ΔESX-1, ΔmmpL7, Δerp, marP::tn, and ΔesxA expressing BFP2, mWasabi, tdTomato, or tdKatushka2 under the control of the msp12 promoter (Cambier et al., 2014b (link); Cosma et al., 2006 (link); Levitte et al., 2016 (link); Osman et al., 2022 (link); Takaki et al., 2013 (link)) were grown at 33°C under hygromycin B (Cambridge Bioscience) or kanamycin (Sigma-Aldrich) selection in Middlebrook 7H9 medium (BD Difco) supplemented with oleic acid, albumin, dextrose, and Tween-80 (Sigma-Aldrich) (Takaki et al., 2013 (link)). M. tuberculosis ΔleuDΔpanCD mc2 6206 expressing msp12:tdTomato was grown at 37°C under hygromycin B and kanamycin selection in Middlebrook 7H9 medium supplemented with oleic acid, albumin, dextrose, Tween-80, catalase, and 0.05 mg/mL L-leucine and 0.024 mg/mL calcium pantothenate (Sigma-Aldrich) (Roca et al., 2019 (link); Sampson et al., 2011 (link)).
4
Osteogenic and Adipogenic Differentiation of hMSCs
5
Yeast Phenotypic Profiling Using Biolog Plates
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Phenotype microarray plates and standard components for yeast phenotypic analysis were obtained from Biolog Inc. (Hayward, CA). Wild type R. toruloides IFO0880 was precultured to log phase in LB broth at 30°C, 200 RPM in 10 mL culture tubes. Cells were centrifuged 5 min at 3000 RCF, 22°C, washed twice in sterile water, then resuspended OD 600 of 0.005 in Biolog inoculation fluid IFY-0 with 1 μM nicotinic acid (Sigma, N4126), 1 μM myo-inositol (Sigma, I5125), 1 μM thiamine HCl (Sigma, T1270), 1 μM p-aminobenzoic acid (Sigma, A9878), and 1 μM calcium pantothenate (Sigma, 21210) plus Biolog dye mix E (a proprietary, tetrazolium-based dye) and 1 μM menadione sodium bisulfite (Sigma, M5750). For nitrogen, phosphorous, and sulfur sources 100 mM glucose was added to the inoculation fluid. Hundred microliters of the cell suspension was added to each well in plates PM1, PM2, PM3, and PM4. Plates were sealed with clear sealing film (Axygen, CTP-103) and incubated for 120 h at 30°C in the dark. Respiration in each condition was detected by measuring reduction of the dye by comparing absorbance at 590 nm to absorbance at 750 nm.
6
Fluorescent Mycobacterial Strains for Research
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WT M. marinum M strains (ATCC #BAA-535) expressing tdTomato, tdKatushka, or EBFP2 under the msp12 promoter (Takaki et al., 2013 (link)) were grown under hygromycin B (Formedium) selection in 7H9 Middlebrook medium (Difco) supplemented with oleic acid, albumin, dextrose, and Tween-80 (Sigma) (Takaki et al., 2013 (link)).
M. tuberculosis ΔleuD ΔpanCD double auxotroph expressing mCherry or GFP under control of the msp12 promoter were grown under hygromycin B (Formedium) and kanamycin selection in Middlebrook 7H9 broth (Difco) supplemented with oleic acid, albumin, dextrose, catalase, Tween-80 (Sigma), 0.05 mg/ml L-leucine and 0.024 mg/ml calcium pantothenate (Sigma) (Sampson et al., 2011 (link)).
M. tuberculosis ΔleuD ΔpanCD double auxotroph expressing mCherry or GFP under control of the msp12 promoter were grown under hygromycin B (Formedium) and kanamycin selection in Middlebrook 7H9 broth (Difco) supplemented with oleic acid, albumin, dextrose, catalase, Tween-80 (Sigma), 0.05 mg/ml L-leucine and 0.024 mg/ml calcium pantothenate (Sigma) (Sampson et al., 2011 (link)).
7
Culturing M. tuberculosis Strains
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M. tuberculosis cells were grown shaking at 170 to 200 rpm at 37°C in Middlebrook 7H9 medium (Difco) supplemented with 10% ADS, 0.2% Casamino Acids (Difco), 0.02% tyloxapol, and 50 μg/mL calcium pantothenate (Sigma). Cells were grown in 10- to 11-mL cultures in closed 30-mL square polyethylene terephthalate copolyester, glycol modified (PETG) bottles (Fisher). ΔgtrRM. tuberculosis strains were grown with 5 μg/mL ALA and/or 25 μM hemin chloride. For heme depletion, ΔgtrR cells were pelleted, washed 1 time in 7H9 medium, and resuspended in 7H9 medium without hemin or ALA supplementation. Sensor strains were grown with 40 μg/mL kanamycin.
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8
Bovine Preadipocyte Isolation and Differentiation
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Adipose tissue from the kidneys was collected under aseptic operation and transported to
the laboratory. Primary cells were obtained from tissues by enzymatic digestion. The
digested cells were cultured in DMEM/F12 (Dulbecco's Modified Eagle Medium and Nutrient
Mixture F-12, HyClone, USA) medium containing 10 % FBS (fetal bovine serum;
Invitrogen, USA). Each 50 mL of maintenance medium was supplemented with
500 of insulin (Abcam, UK), 1.65 of calcium
pantothenate (Sigma, USA), and 8.5 of bovine transferrin (Sigma, USA).
After 7 d of induced differentiation, cells were stained with Oil Red O (Sigma, USA) and
observed under the microscope (Olympus, CKX41, Japan) until the round and bright lipid
droplets had formed in the cytoplasm. Total RNA was extracted from bovine preadipocytes
using TRIzol reagent. One microgram total RNA was reverse transcribed into cDNA to study
the effect of expression of ACSL5 gene on the triglycerides content in
preadipocytes.
the laboratory. Primary cells were obtained from tissues by enzymatic digestion. The
digested cells were cultured in DMEM/F12 (Dulbecco's Modified Eagle Medium and Nutrient
Mixture F-12, HyClone, USA) medium containing 10 % FBS (fetal bovine serum;
Invitrogen, USA). Each 50 mL of maintenance medium was supplemented with
500 of insulin (Abcam, UK), 1.65 of calcium
pantothenate (Sigma, USA), and 8.5 of bovine transferrin (Sigma, USA).
After 7 d of induced differentiation, cells were stained with Oil Red O (Sigma, USA) and
observed under the microscope (Olympus, CKX41, Japan) until the round and bright lipid
droplets had formed in the cytoplasm. Total RNA was extracted from bovine preadipocytes
using TRIzol reagent. One microgram total RNA was reverse transcribed into cDNA to study
the effect of expression of ACSL5 gene on the triglycerides content in
preadipocytes.
9
Chemically Defined Media for Cladode Fermentation
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A chemically defined medium containing a sugar mixture similar in composition to the enzymatic hydrolysate of the O. ficus-indica cladodes was initially used to evaluate the performance of both yeast strains as a benchmark before proceeding with the fermentation of the actual cladode hydrolysate. This medium contained (per litre) 0.5 g citric acid, 5 g (NH4)2HPO4, 0.75 g KH2PO4, 0.5 g K2HPO4, 0.2 g MgSO4·7H2O, 0.02 g CaCl2·2H2O, 0.1 g NaCl, 1 ml of a trace elements stock solution as described above and 4 ml of a vitamin stock solution. The latter contained (per litre) 0.025 g biotin, 0.5 g calcium pantothenate, 0.5 g nicotinic acid, 0.1 g p-aminobenzoic acid, 0.5 g pyridoxine hydrochloride, 0.5 g thiamine hydrochloride, and 12.5 g m-inositol (all vitamins from Sigma-Aldrich). The sugar mixture was autoclaved separately, whereas the mineral salts solution was autoclaved in the bioreactor at 121 °C for 20 min after adjustment to pH 5.0 by titration with 3 M KOH. The filter-sterilized vitamin stock solution was added to the sterile sugar mixture and aseptically transferred into the bioreactor.
10
Comprehensive Characterization of Energy Drinks
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