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Cd62l

Manufactured by Miltenyi Biotec
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Sourced in Italy

CD62L is a cell surface glycoprotein that functions as a cellular adhesion molecule. It plays a role in the homing and migration of leukocytes.

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Lab products found in correlation

Human serum albumin (hsa), by Thermo Fisher Scientific (1 mentions) Phenol red, by Thermo Fisher Scientific (1 mentions) Glutamine, by Thermo Fisher Scientific (1 mentions) Human serum albumin (hsa), by Merck Group (1 mentions) Hank s balanced salt solution, by Thermo Fisher Scientific (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) Csa detection kit, by Miltenyi Biotec (1 mentions) Cd11b, by Miltenyi Biotec (1 mentions) Ghost dye violet 510, by BioLegend (1 mentions)

Close spelling variants of this product

CD62L microbeads CD62L-PE-Vio770 CD62L-Vioblue CD62L-FITC Mouse CD4+CD62L+ T Cell Isolation Kit II Anti‐CD62L‐PE CD4+CD62L+ isolation kit CD4+CD62L+ T Cell Isolation Kit II CD4+CD62L+ T cell isolation kit CD62L-PE

3 protocols using cd62l

1

Isolation and Characterization of Human Polymorphonuclear Leukocytes

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Human peripheral blood polymorphonuclear leukocytes (PMNs) were isolated from healthy donors (in accordance with relevant national guidelines and regulations, and informed consent of healthy volunteers: GS1-EK-4/543–2018) using EasySep Direct Human Pan-Granulocyte Isolation Kit (Stemcell). Purity and non-activated status of PMNs were validated using flow cytometry (FSC, SSC, CD1 lb, CD 14, CD45, CD62L, CD66b, CD235a; Miltenyi). For short-term storage or staining, PMNs were kept in Hanks balanced salt solution without phenol red (Gibco), supplemented with 0.6% human serum albumin (Sigma) and 1% glutamine (Gibco), with gently agitation in a ventilated tube in a humidified incubator. For experiments, PMNs were resuspended in RPMI without phenol red, supplemented with 0.6% human serum albumin, 20 mM HEPES (Gibco) and 1% glutamine.
Renkawitz J., Kopf A., Stopp J., de Vries I., Driscoll M.K., Merrin J., Hauschild R., Welf E.S., Danuser G., Fiolka R, & Sixt M. (2019). Nuclear positioning facilitates amoeboid migration along the path of least resistance. Nature, 568(7753), 546-550.
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2

Characterization of MM-specific T Cells

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A flow cytometry-based, phenotypic, and functional characterization of IFNγ-producing MM-specific T cells was carried out in two selected patients with robust responses in the ELISpot assay, by applying the CSA Detection Kit (Miltenyi Biotec, Bologna, Italy), according to the manufacturer’s instructions and protocols we previously described [34 (link),35 (link)], using, as specific antigenic stimulation, the 10 MMAA-derived peptide pools, individually. T-cell phenotype and memory profile of cytokine-producing lymphocytes were assessed after sample counterstaining with monoclonal antibodies for flow cytometry, including: CD3, CD8, CD4, CD62L, and CCR7 (Miltenyi Biotec, Bologna, Italy).
Lagreca I., Nasillo V., Barozzi P., Castelli I., Basso S., Castellano S., Paolini A., Maccaferri M., Colaci E., Vallerini D., Natali P., Debbia D., Pirotti T., Ottomano A.M., Maffei R., Bettelli F., Giusti D., Messerotti A., Gilioli A., Pioli V., Leonardi G., Forghieri F., Bresciani P., Cuoghi A., Morselli M., Manfredini R., Longo G., Candoni A., Marasca R., Potenza L., Tagliafico E., Trenti T., Comoli P., Luppi M, & Riva G. (2023). Prognostic Relevance of Multi-Antigenic Myeloma-Specific T-Cell Assay in Patients with Monoclonal Gammopathies. Cancers, 15(3), 972.
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3

Comprehensive Flow Cytometry Analyses

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Flow cytometric analyses were performed as previously described [57 (link)]. eBioscience antibodies were used unless otherwise specified: TCRβ (H57–597), CD4 (RM4–5), CD8 (53–6.7), CD19 (eBio1D3), CD45 (30-F11), CD11b (M1/70), CD44 (IM7), CD62L (MEL14), CD11c (N418), Ly6G (1A8), PDGFRα (APA5), O4 (O4; Miltenyi), CD13 (R3–242; BD), CD31 (390; BioLegend), MHC1 (28–8-6; BioLegend), and CD16/CD32 (93). Viability was assessed using a Zombie Aqua Fixable Viability kit (cat. no. 423101, BioLegend) or Ghost Dye Violet 510 (cat. no. 13–0870, Tonbo Biosciences).
Fernández-Castañeda A., Chappell M.S., Rosen D.A., Seki S.M., Beiter R.M., Johanson D.M., Liskey D., Farber E., Onengut-Gumuscu S., Overall C., Dupree J.L, & Gaultier A. (2019). The active contribution of OPCs to neuroinflammation is mediated by LRP1. Acta neuropathologica, 139(2), 365-382.
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