Cells were labelled with primary OV6 antibody (mouse IgG1; R&D Systems, Minneapolis), magnetically tethered to rat anti-mouse IgG1 microbeads, and sorted with a Mini-MACS™ Cell Sorter Kit (Miltenyi Biotec, CA). All of the procedures were following the manufacturer’s instructions. The sorted cells were evaluated by flow cytometry analysis. The flow cytometry was performed with MoFlo Sorter (Beckman, Brea, CA) using an APC-conjugated-OV6 antibody (R&D Systems, Minneapolis) and following manufacturer’s instruction.
Mini macs cell sorter kit
Manufactured by Miltenyi Biotec
Sourced in Canada
The Mini-MACS™ Cell Sorter Kit is a compact and efficient cell sorting system. It utilizes magnetic cell separation technology to isolate specific cell populations from complex samples. The kit provides the necessary components for performing cell sorting procedures.
Automatically generated - may contain errors
2 protocols using mini macs cell sorter kit
1
Enrichment and Characterization of OV6+ Cells
2
Magnetically Sorted OV6+ Cells
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Cells were labelled with primary OV6 antibody (mouse IgG1; R&D Systems, Minneapolis), magnetically tethered to rat anti-mouse IgG1 microbeads, and sorted with a Mini-MACS™ Cell Sorter Kit (Miltenyi Biotec, CA). All of the procedures were following the manufacturer’s instructions. The sorted cells were evaluated by flow cytometry analysis. The flow cytometry was performed with MoFlo Sorter (Beckman, CA) or ImageStreamx (Millipore, US) using an APC-conjugated-OV6 antibody (R&D Systems, Minneapolis) and following manufacturer’s instruction.
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