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Lipidyzer internal standard mix

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The Lipidyzer Internal Standard Mix is a collection of known lipid compounds used as internal standards for the quantification of lipids in biological samples. The mix contains a variety of lipid classes, including but not limited to, glycerolipids, glycerophospholipids, sphingolipids, and sterols. This product is designed to be used as a reference standard to ensure the accuracy and reliability of lipid analysis.

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Lab products found in correlation

Selexion tuning kit, by AB Sciex (3 mentions) Robovials, by Thermo Fisher Scientific (3 mentions) Sciex lipidyzer platform, by AB Sciex (2 mentions) Omni bead ruptor elite, by Omni International (1 mentions) Lipidyzer platform, by AB Sciex (1 mentions)

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Lipidyzer (8 citations)

3 protocols using lipidyzer internal standard mix

1

Targeted Lipidomic Analysis of Plasma

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Lipids from 25 ul of plasma were extracted using a modified Bligh and Dyer extraction method by the UCLA lipidomics core facility56 . Prior to biphasic extraction, a 13-lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers were dried down in a Genevac EZ-2 Elite evaporator. Lipid samples were resuspended in 1:1 methanol/dichloromethane with 10mM ammonium acetate and transferred to robovials (Thermo 10800107) for analysis. Samples were analyzed on the Sciex Lipidyzer Platform for targeted quantitative measurement of 1100 lipid species across 13 classes. Differential Mobility Device on Lipidyzer was tuned with SelexION tuning kit (Sciex 5040141). Instrument settings, tuning settings, and MRM list are available upon request.
Fung T.C., Vuong H.E., Luna C.D., Pronovost G.N., Aleksandrova A.A., Riley N.G., Vavilina A., McGinn J., Rendon T., Forrest L.R, & Hsiao E.Y. (2019). Intestinal serotonin and fluoxetine exposure modulate bacterial colonization in the gut. Nature microbiology, 4(12), 2064-2073.
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2

Quantitative Lipid Profiling of Liver

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A total of 50–100 mg of frozen liver are homogenized in the Omni Bead Ruptor Elite with 2 mL homogenizer tube system (Omni, 19-628D). Samples are homogenized in cold phosphate-buffered saline (PBS) for 3 cycles of 10 s each at 5 m/s with a 10 s dwell time between cycles. A total of 3–6 mg of homogenized material are applied to a modified Bligh and Dyer extraction26 (link). Prior to biphasic extraction, a 13 lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers are dried down in a Genevac EZ-2 Elite. Lipid samples are resuspended in 1:1 methanol/dichloromethane with 10 mM ammonium acetate and transferred to robovials (Thermo 10800107) for analysis.
Samples are analyzed on the Sciex Lipidyzer Platform for targeted quantitative measurement of 1100 lipid species across 13 lipid sub-classes. Differential Mobility Device on Lipidyzer is tuned with SelexION tuning kit (Sciex 5040141). Instrument settings, tuning settings, and MRM list available upon request. Data analysis performed on Lipidyzer software. Quantitative values are normalized to milligrams of material used.
Zhang Z., Feng A.C., Salisbury D., Liu X., Wu X., Kim J., Lapina I., Wang D., Lee B., Fraga J., Pan C., Williams K.J., Lusis A.J., Scumpia P, & Sallam T. (2020). Collaborative interactions of heterogenous ribonucleoproteins contribute to transcriptional regulation of sterol metabolism in mice. Nature Communications, 11, 984.
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3

Targeted Lipidomic Analysis of Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lipids from 25 ul of plasma were extracted using a modified Bligh and Dyer extraction method by the UCLA lipidomics core facility56 . Prior to biphasic extraction, a 13-lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers were dried down in a Genevac EZ-2 Elite evaporator. Lipid samples were resuspended in 1:1 methanol/dichloromethane with 10mM ammonium acetate and transferred to robovials (Thermo 10800107) for analysis. Samples were analyzed on the Sciex Lipidyzer Platform for targeted quantitative measurement of 1100 lipid species across 13 classes. Differential Mobility Device on Lipidyzer was tuned with SelexION tuning kit (Sciex 5040141). Instrument settings, tuning settings, and MRM list are available upon request.
Fung T.C., Vuong H.E., Luna C.D., Pronovost G.N., Aleksandrova A.A., Riley N.G., Vavilina A., McGinn J., Rendon T., Forrest L.R, & Hsiao E.Y. (2019). Intestinal serotonin and fluoxetine exposure modulate bacterial colonization in the gut. Nature microbiology, 4(12), 2064-2073.
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