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2 protocols using anti arnt

1

Reagents for Hypoxia Signaling Pathway

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Anti-SMYD3 (#ab199361) antibody was purchased from Abcam. Antibodies including anti-HIF1α (#36169), anti-VHL (#68547), anti-Histone H3 (#4499), anti-HIF2α (#7096), anti-ARNT (#5537), and normal rabbit IgG (#2729) were purchased from Cell Signaling Technology. Anti-ACTB (#AC026) antibody was purchased from ABclonal. Anti-HA (#901515) antibody was purchased from Covance. Anti-Myc (#SC-40) and anti-GAPDH (#SC-477242) antibodies were purchased from Santa Cruz Biotechnology. Anti-α-tubulin (#62204), Alexa Fluor 488 goat anti-rabbit IgG (#A11008), Alexa Fluor 594 goat anti-mouse IgG (#A11005), CM-H2DCFDA (#C6827), and MitoSOX Red (#M36008) were purchased from Thermo Fisher Scientific. CoCl2 (#C8661) and deferoxamine mesylate salt (#D9533) were purchased from Sigma. FG4592 (#S1007) and PX478 (#S7612) were purchased from Selleck. Cycloheximide (#HY-12320) was purchased from MCE.
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2

Protein Interaction Detection Protocol

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Anti-USP38 antibody (#17767-1-AP) was purchased from Proteintech. Antibodies including anti-HIF1α (#36169), anti-VHL (#68547), anti-ARNT (#5537), anti-Ubiquitin (#3936), and normal rabbit IgG (#2729) were purchased from Cell Signaling Technology. Anti-ACTB antibody (#AC026) was purchased from ABclonal. Anti-HA antibody (#901515) was purchased from Covance. Anti-Myc antibody (#SC-40) was purchased from Santa Cruz Biotechnology. Image-iT Green Hypoxia Reagent (#I14834) was purchased from Thermo Fisher Scientific. CoCl2 (#C8661), MG-132 (#474790), anti-Flag antibody (#F1804) and anti-Flag antibody-conjugated agarose beads (#A2220) were purchased from Sigma. Protein G Sepharose (#17-0618-01) was purchased from GE HealthCare Company. PAX478 (#S7612) was purchased from Selleck.
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