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K 982

Manufactured by R&D Systems
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The K-982 is a compact, high-precision laboratory centrifuge designed for a variety of applications. It features a robust, durable construction and can accommodate a range of sample volumes and tube sizes. The centrifuge operates at adjustable speeds up to 6,000 rpm, providing efficient separation of samples. This product is suitable for use in research, clinical, and industrial settings where reliable and consistent centrifugation is required.

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Lab products found in correlation

Strep tag 2 antibody hrp conjugate, by Merck Group (2 mentions) Ube2d1, by R&D Systems (2 mentions) Mgatp solution, by R&D Systems (2 mentions)

2 protocols using k 982

1

Identifying compatible E2 enzymes for BCL6 ubiquitination

Check if the same lab product or an alternative is used in the 5 most similar protocols
In vitro ubiquitination for identification of compatible E2 conjugating enzymes was performed by following the manufacturer’s instruction (K-982, Boston Biochem), using Strep II-Avi-BCL65−360 and Flag-SIAH1FL. Time-course in vitro ubiquitination was performed by mixing the substrate (BCL6, 2 μM), E3 (SIAH, 0.5 μM), E1 (UBA1, Boston Biochem, 0.2 μM, E2 (UBE2D1, Boston Biochem, 0.5 μM), and ubiquitin (Boston Biochem, 50 μM), with a reaction buffer (B-71, Boston Biochem) containing BI-3802 or DMSO (normalized to 1% DMSO) in 15 μL volume each. Reactions were initiated by adding 5 μL of Mg-ATP solution (B-20, Boston Biochem), incubated for up to 60 min at 37 ºC, and analyzed by western blot using Strep tag II Antibody HRP conjugate (71591–3, Sigma) at 1:4,000.
Słabicki M., Yoon H., Koeppel J., Nitsch L., Roy Burman S.S., Di Genua C., Donovan K.A., Sperling A.S., Hunkeler M., Tsai J.M., Sharma R., Guirguis A., Zou C., Chudasama P., Gasser J.A., Miller P.G., Scholl C., Fröhling S., Nowak R.P., Fischer E.S, & Ebert B.L. (2020). Small molecule-induced polymerization triggers degradation of BCL6. Nature, 588(7836), 164-168.
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2

Identifying compatible E2 enzymes for BCL6 ubiquitination

Check if the same lab product or an alternative is used in the 5 most similar protocols
In vitro ubiquitination for identification of compatible E2 conjugating enzymes was performed by following the manufacturer’s instruction (K-982, Boston Biochem), using Strep II-Avi-BCL65−360 and Flag-SIAH1FL. Time-course in vitro ubiquitination was performed by mixing the substrate (BCL6, 2 μM), E3 (SIAH, 0.5 μM), E1 (UBA1, Boston Biochem, 0.2 μM, E2 (UBE2D1, Boston Biochem, 0.5 μM), and ubiquitin (Boston Biochem, 50 μM), with a reaction buffer (B-71, Boston Biochem) containing BI-3802 or DMSO (normalized to 1% DMSO) in 15 μL volume each. Reactions were initiated by adding 5 μL of Mg-ATP solution (B-20, Boston Biochem), incubated for up to 60 min at 37 ºC, and analyzed by western blot using Strep tag II Antibody HRP conjugate (71591–3, Sigma) at 1:4,000.
Słabicki M., Yoon H., Koeppel J., Nitsch L., Roy Burman S.S., Di Genua C., Donovan K.A., Sperling A.S., Hunkeler M., Tsai J.M., Sharma R., Guirguis A., Zou C., Chudasama P., Gasser J.A., Miller P.G., Scholl C., Fröhling S., Nowak R.P., Fischer E.S, & Ebert B.L. (2020). Small molecule-induced polymerization triggers degradation of BCL6. Nature, 588(7836), 164-168.
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