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3 protocols using n alkane mixed standard c7 c30

1

Extraction and Analysis of Lipids

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Sodium chloride (NaCl) was purchased from Sangong Biotech Co., Ltd (Shanghai, China). Analytical grade pure n-hexane and ethyl acetate were purchased from Tianjin Fuyu Fine Chemical Co., Ltd (Tianjin, China). The analytical reagent toluene and 10% boron trifluoride-methanol solution were obtained separately from Guangzhou Chemical Reagent Factory (Guangzhou, China) and Shanghai Macklin Biochemical Technology Co., Ltd (Shanghai, China). The S-(+)-2-octanol (98%) and heptadecanoic acid (98%) were purchased from Shanghai Yuanye Biotechnology Co., Ltd (Shanghai, China). n-Alkane mixed standard (C7-C30) of chromatographic grade was purchased from Sigma Aldrich (St. Louis, MO, USA).
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2

Comprehensive Metabolite Analysis Protocol

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N,N-dimethylformamide, acetonitrile, acetic acid, and methanol (chromatographic grade, Shanghai National Pharmaceutical Group Reagent Co, Shanghai, China). Methanol, sodium chloride, sodium carbonate, folin-phenol, disodium hydrogen phosphate, potassium dihydrogen phosphate, ninhydrin, stannous chloride, aluminum trichloride, anthrone, anhydrous dextrose, concentrated sulfuric acid (analytical grade), Shanghai National Pharmaceutical Group Reagent Co, Shanghai China. Epicatechin (EC), epigallocatechin (EGC), catechin (D, L-C), epigallocatechin gallate (EGCG), epigallocatechin gallate (ECG), gallocatechin gallate (GCG), aspartic acid, serine, glutamic acid, glycine, histidine, arginine, threonine, alanine, proline, theanine, cysteine, tyrosine, valine, methionine, lysine, isoleucine, leucine, phenylalanine standard, n-alkane mixed standard (C7~C30), Sigma-Aldrich Corp., St. Louis, MO, USA. Ethyl decanoate (99%), Shanghai Aladdin Biochemical Technology Co, Shanghai, China.
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3

Volatile Compounds Extraction and Characterization

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Anhydrous sodium sulfate, ethanol, and dichloromethane were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). 2,4,6-Trimethylpyridine, hexanal, nonanal, (E)-2-nonenal, 1-penten-3-ol, 3-octanone, 2-pentylfuran, 1-butanol, octanal, (E)-2-octenal, 2,3-octanedione, (E,Z)-2,6-nonadienal, (E)-2-pentenal, 1-octen-3-ol, (E,E)-2,4-heptadienal, heptanal, benzaldehyde, lactic acid (85 %), (E,E)-2,4-nonadienal, (E)-2-hexenal, (E)-2-decenal, 2,4-di-tert-butylphenol, (E,E)-2,4-decadienal, acetic acid and n-Alkane mixed standard (C7-C30) of chromatographic grade was purchased from Sigma Aldrich (St. Louis, MO, USA).
This study employed GC–MS model GC-14B (Shimadzu, Kyoto, Japan), SDE device model HB-TSZL (Anhui Great Wall Glass Instrument Factory (Xuancheng, China), and the thermoelectric thermostatic water bath model HWS-24 from the Shanghai Yiheng Scientific Instrument Co., LTD (Shanghai, China).
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