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Buffer salts

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Buffer salts are a class of chemical compounds that are used to maintain a specific pH level in aqueous solutions. They are commonly used in various laboratory applications, including biochemistry, molecular biology, and analytical chemistry. Buffer salts help to stabilize the pH of solutions, ensuring that the desired pH conditions are maintained during experiments or processes.

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Lab products found in correlation

Soybean pi, by Avanti Polar Lipids (3 mentions) Cholesterol, by Merck Group (3 mentions) Dimyristoylphosphatidylcholine dmpc, by Avanti Polar Lipids (2 mentions) Ultra pure nitric acid, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline solution, by Thermo Fisher Scientific (1 mentions) Tryptic soy broth (tsb), by Teknova (1 mentions) Horseradish peroxidase conjugated anti mouse igg antibody, by Merck Group (1 mentions) 3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (1 mentions) Dimyristoylphosphatidylcholine, by Avanti Polar Lipids (1 mentions)

Spelling variants of this product

Hank’s balanced salt solution (HBSS) buffer (7 citations) High-Salt Elution Buffer (3 citations) Hank's Balanced Salt Solution buffer (3 citations) Hank’s buffered salt solution (HBSS) buffer (2 citations) HEPES-Hank’s Balanced Salt Solution (HBSS) buffer (2 citations)

4 protocols using buffer salts

1

Purification and Characterization of rcFVIII

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rcFVIII was expressed, purified, and characterized as previously described15 (link). The specific activity of the protein was 2.25 U/μg. Dimyristoylphosphatidylcholine (DMPC) and soybean PI were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Cholesterol was purchased from Sigma-Aldrich (St.Louis, Missouri, USA). FVIII chromogenic assay detection kits were purchased from DiaPharma (Westchester, Ohio). Control plasmas and activated partial thromboplastin time (aPTT) reagents were purchased from Precision Biologics (Dartmouth, Canada) and Tcoag (Parsippany, NJ) respectively. Buffer salts were purchased from Fisher Scientific.
Shetty K.A., Merricks E.P., Raymer R., Rigsbee N., Nichols T.C, & Balu-Iyer S.V. (2016). Soy phosphatidylinositol containing lipid nanoparticle prolongs the plasma survival and hemostatic efficacy of B-domain deleted recombinant canine Factor VIII in Hemophilia A dogs. Journal of pharmaceutical sciences, 105(8), 2459-2464.
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2

Photocrosslinkable Polymer-Fabric Composites

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The ZnTMPyP4+ tetrachloride PS was purchased from Frontier Scientific, while MB and RB were obtained from Acros Organics. The photocrosslinkable SbQ-PVA polymer with 4.1 mol% functional SbQ groups was supplied by Polysciences, Inc. The following materials were donated by Vescom America: Capri (polyester curtain fabric), Deans (polyester upholstery), Husk (proprietary wallcovering), PRU-86364 (proprietary wallcovering), and Wolin (a 75/20/5 w/w/w/ wool/polyester/polyamide upholstery combination). Buffer salts for the preparation of phosphate-buffered saline (PBS) solution and ultrapure nitric acid for ICP-OES analysis were purchased from Fisher Scientific. Tryptic soy broth was purchased from Teknova, and all media and buffer solutions were prepared in ultrapure water provided by an Easypure II system (Barnstead).
Ghareeb C.R., Peddinti B.S., Kisthardt S.C., Scholle F., Spontak R.J, & Ghiladi R.A. (2021). Toward Universal Photodynamic Coatings for Infection Control. Frontiers in Medicine, 8, 657837.
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3

Purification and Characterization of FVIII

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BDD FVIII was expressed and purified in the lab of Dr. Philip Fay as previous described 26 (link)–28 (link). Specific activity of the protein was 6.5 IU/µg. Dimyristoylphosphatidylcholine (DMPC) and soybean PI were purchased from Avanti Polar Lipids (Alabaster, Alabama, USA). Cholesterol was purchased from Sigma-Aldrich (St.Louis, Missouri, USA). FVIII chromogenic assay detection kits were purchased from Chromogenix (Chapel Hill, NC). Control plasmas and activated partial thromboplastin (aPTT) reagents were purchased from Precision Biologics (Dartmouth, Canada) and Tcoag (Parsippany, NJ) respectively. Monoclonal antibody ESH8 was purchased from American Diagnostica Inc. (Greenwich, CT). Alkaline phosphatase conjugates of goat anti-mouse IgG/IgM was obtained from Southern Biotechnology Associates, Inc. (Birmingham, AL). Buffer salts were purchased from Fisher Scientific.
Shetty K.A., Kosloski M.P., Mager D.E, & Balu-Iyer S.V. (2014). Soy phosphatidylinositol containing nanoparticle prolongs hemostatic activity of B-domain deleted Factor VIII in Hemophilia A mice. Journal of pharmaceutical sciences, 104(2), 388-395.
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4

FVIII Expression and Purification Protocol

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The BDD FVIII was expressed and purified in the laboratory of Dr Philip Fay as described previously [28 (link)–30 (link)]. The specific activity of the protein was 6.5 IU/µg. Dimyristoylphosphatidylcholine (DMPC) and soybean PI were purchased from Avanti Polar Lipids (Alabaster, Alabama, USA). Cholesterol was purchased from Sigma-Aldrich (St Louis, MI, USA). Two-stage chromogenic assay kits for FVIII activity were purchased from Chromogenix (Chapel Hill, NC). Murine anti-FVIII monoclonal antibody ESH-8 was purchased from American Diagnostica (Stamford, CT). Horseradish peroxidase conjugated anti-mouse IgG antibody and 3,3′,5,5′-tetramethylbenzidine were purchased from Sigma-Aldrich (St Louis, MO). Control plasmas and activated partial thromboplastin time (aPTT) reagents were purchased from Precision Biologics (Dartmouth, Canada) and Tcoag (Parsippany, NJ). Buffer salts were purchased from Fisher Scientific.
Shetty K.A., Kosloski M.P., Mager D.E, & Balu-Iyer S.V. (2016). Factor VIII associated with lipidic nanoparticles retains efficacy in the presence of anti-factor VIII antibodies in hemophilia A mice. Biopharmaceutics & drug disposition, 37(7), 409-420.
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