Granzyme b

Manufactured by Akoya Biosciences

Sourced in United States

Granzyme B is a serine protease enzyme found in the cytotoxic granules of natural killer cells and cytotoxic T lymphocytes. It plays a key role in the induction of apoptosis in target cells during the cell-mediated immune response.

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Lab products found in correlation

Cd8 clone 4b11, by Leica (1 mentions) Cd68 clone kp1, by Agilent Technologies (1 mentions) Pd l1 clone 22c3, by Agilent Technologies (1 mentions) Ncl l cd8 4b11, by Leica (1 mentions)

2 protocols using granzyme b

Multiplex Immunofluorescence Tissue Analysis

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Tissue mIF was conducted by Opal Polaris 5color IHC staining kit including PD‐L1, CD4+ T cells, CD8+ T cells, FoxP3+ regulatory T cells and Granzyme B (Akoya Biosciences) according to the manufacturer's protocol. The whole slide image was scanned and representative 5 to 7 regions of interest were chosen by the pathologist at ×200 resolution as multispectral images. Image analysis was performed using the InForm 2.4.8 Image Analysis Software.

Yu F., Peng M., Bai J., Zhu X., Zhang B., Tang J., Liu W., Chen C., Wang X., Chen M., Tan S., Sun Y., Liang Q., Li J., Hu Y., Liao A., Hu H., He Y., Xiao X., Wang B., Xing G., Xu Y., Chen R., Xia X, & Chen X. (2022). Comprehensive characterization of genomic and radiologic features reveals distinct driver patterns of RTK/RAS pathway in ground‐glass opacity pulmonary nodules. International Journal of Cancer, 151(11), 2020-2030.

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Multiplex IHC Profiling of Tumor-Infiltrating Immune Cells

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Immunohistochemical (IHC) staining using whole section FFPE tissues was performed with the following primary antibodies: CD8 (clone 4B11, 1:400, Cat#NCL-L-CD8-4B11, Leica Biosystems, Newcastle, UK), granzyme B (clone 11F1, 1:128, Cat#NCL-L-GRAN-B, Leica Biosystems, Newcastle, UK), CD68 (clone KP1, 1:2000, Cat#M0814, Dako, Glostrup, Denmark), and PD-L1 (clone 22C3, 1:50, Cat#M3653, Dako, Glostrup, Denmark). PD-L1 expression was evaluated as the tumor proportion score (TPS), which was defined as the percentage of tumor cells with PD-L1 expression, and the combined positive score (CPS), which was defined as the number of PD-L1-expressing tumors and immune cells (lymphocytes and macrophages) divided by the number of all tumor cells and multiplied by 100. To quantify CD8-, CD68-, and granzyme B-positive immune cells, up to 10 representative images of each IHC slide were acquired and analyzed by two expert genitourinary pathologists (S.H. and G.Y.K.) using the inForm 2.6 software (Akoya Biosciences, Marlborough, MA, USA). Positive cells were scored out of the total number of cells counted in representative images.

Lee J.H., Hwang S., Jee B., Kim J.H., Lee J., Chung J.H., Song W., Sung H.H., Jeon H.G., Jeong B.C., Seo S.I., Jeon S.S., Lee H.M., Park S.H., Kwon G.Y, & Kang M. (2023). Fat Loss in Patients with Metastatic Clear Cell Renal Cell Carcinoma Treated with Immune Checkpoint Inhibitors. International Journal of Molecular Sciences, 24(4), 3994.

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