For co-immunoprecipitation assays, 35S:ABI4-GFP and 35S:HDA9-HA expression cassettes were co-infiltrated into leaves of N. benthamiana, and after 3 day of incubation, total protein was extracted from the leaves, pulled down with α-GFP, and immunoblotted with α-HA. Each immunoblot was incubated with the appropriate primary antibody (α-HA antibody, 1:2000; α-GFP antibody) for 2 h at room temperature or overnight at 4°C. The membranes were developed using peroxidase-conjugated secondary antibody: 1:2000 for α-rabbit antibody (GE, Little Chalfont, Buckinghamshire, United Kingdom) and 1:1000 for α-rat IgG (Sigma, St. Louis, MO, United States).
α rat igg
Manufactured by Merck Group
Sourced in Macao
The α-rat IgG is a laboratory reagent used for the detection and quantification of rat immunoglobulin G (IgG) in various immunoassay applications. It is a purified polyclonal antibody raised in a suitable animal species against rat IgG.
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Lab products found in correlation
3 protocols using α rat igg
1
Co-Immunoprecipitation of ABI4 and HDA9
2
ICOS-ICOSL Signaling Blockade in Mice
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α-IcosL Ab was obtained from BioXCell (BE0028), and α-Rat IgG was obtained from Sigma Millipore. To block ICOS-ICOSL signaling, 200μg of α-IcosL was administered in sterile 1 × PBS every other day to WT mice beginning on the day before re-challenge (day 89 p.i.). α-Rat IgG was administered in the same manner to control WT mice.
3
Fly Protein Extraction and Western Blotting
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Protein extractions from Drosophila S2 cells and adult fly heads, western blotting, and image analysis were performed as previously described (10, 11) . Primary antibodies: a-HA 3F10 (Roche, Indianapolis, IN) at 1:2000 for TIM-HA, a-V5 (Thermo Fisher Scientific) at 1:3000 for PER-V5, a-FLAG (Sigma) at 1:7000 for XPO1-FLAG, a-TIM (R5839, RRID:AB_2782953) at 1:1000 for TIM (12) , a-pS1404 (RB S4602-2, RRID:AB_2814716) at 1:2000 for TIM(pS1404) isoforms, a-CLK (GP6139, RRID:AB_2827523) at 1:2000 for CLK, a-PER (GP5620; RRID:AB_2747405) at 1:2000 for PER and a-HSP70 (Sigma) at 1:10000 was used for to indicate equal loading and for normalization. Secondary antibodies conjugated with HRP were added as follows: α-mouse IgG (Sigma) at 1:2000 for α-V5 detection, 1:2000 for α-FLAG detection, or 1:10,000 for α-HSP70 detection, α-guinea pig IgG (Sigma) at 1:1000 for α-PER detection, a-rabbit IgG (Sigma) at 1:2000 for a-pS1404 detection ,and α-rat IgG (Sigma) at 1:1000 for detecting α-HA and a-TIM.
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