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Mouse monoclonal anti mbp

Manufactured by New England Biolabs
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Mouse monoclonal anti-MBP is an antibody reagent produced by New England Biolabs. It is a purified mouse monoclonal antibody that specifically recognizes the Myelin Basic Protein (MBP) antigen.

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Lab products found in correlation

Hrp conjugated mouse monoclonal anti flag m2, by Merck Group (4 mentions) Irdye 800cw goat anti mouse igg, by LI COR (4 mentions) Mouse monoclonal anti gfp, by BioLegend (2 mentions) Peroxidase conjugated anti mouse igg, by Dianova (2 mentions) Hrp conjugated goat anti rabbit igg, by Bio-Rad (2 mentions) Hrp conjugated goat anti mouse igg, by Bio-Rad (2 mentions) Mouse monoclonal anti flag m2, by Merck Group (2 mentions) Anti lexa, by Santa Cruz Biotechnology (2 mentions) Hrp conjugated donkey anti goat igg, by Santa Cruz Biotechnology (2 mentions) Hrp conjugated donkey anti rat igg, by Abcam (2 mentions) Rabbit polyclonal anti kar2 y 115, by Santa Cruz Biotechnology (2 mentions) Irdye 680 goat anti rabbit igg, by LI COR (2 mentions) Mouse monoclonal anti myc, by Abcam (2 mentions) Odyssey infrared imaging system, by LI COR (2 mentions) Anti trfp, by Evrogen (1 mentions)

Close spelling variants of this product

Mouse monoclonal anti-MBP antibody Mouse anti-MBP Anti-MBP

6 protocols using mouse monoclonal anti mbp

1

Antibody Generation and Characterization for Jacob

Cited 2 times
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Custom-made, affinity-purified antibodies generated against two short amino acid stretches of rat Jacob (285–299 and 400–314, Jb150) have been described previously [14 (link), 21 (link), 22 (link)]. The following commercial primary antibodies were used in the present study: mouse monoclonal anti-MAP2 (Sigma-Aldrich, M4403), anti-MAP2, Alexa Fluor 488 conjugated (Merck, MAB 3418x), mouse monoclonal anti-LaminB1 (ProteinTeck, 3C10G12), rabbit polyclonal anti-CRM1 (ProteinTeck), mouse monoclonal anti-MBP (NEB), mouse monoclonal anti-GFP (BioLegend, B34), and rabbit polyclonal anti-tRFP (EVROGEN). For imaging and WB analysis, the following secondary antibodies were used: anti-rabbit, anti-mouse secondary antibodies conjugated with Alexa Fluor 488, 561 or 647 (Life Technologies), peroxidase-conjugated anti-mouse IgG (Dianova) and anti-rabbit IgG (Dianova).
Samer S., Raman R., Laube G., Kreutz M.R, & Karpova A. (2021). The nuclear lamina is a hub for the nuclear function of Jacob. Molecular Brain, 14, 9.
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2

Western Blot Analysis of Protein Extracts

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Denaturing whole-cell extracts were prepared in10% trichloroacetic acid by agitation with glass beads. Proteins were solubilized in Laemmli sample buffer, separated by SDS-PAGE and analyzed by western blotting. Antibodies were goat polyclonal anti-LexA (1:2000, Santa Cruz), rat polyclonal anti-α-tubulin (1:5000, Bio-rad), mouse monoclonal anti-Flag M2 (1:2000, Sigma) HRP-conjugated mouse monoclonal anti-Flag M2 (1:2000, Sigma), mouse monoclonal anti-MBP (1:2000, NEB), rabbit polyclonal anti-Spo11 (1:1000, this laboratory). Secondary antibodies were used at 1:5000 dilution: IRDye 800CW goat anti-mouse IgG (LI-COR), HRP-conjugated goat anti-mouse IgG (Bio-Rad), HRP-conjugated goat anti-rabbit IgG (Bio-Rad), HRP-conjugated donkey anti-goat IgG (Santa Cruz), HRP-conjugated donkey anti-rat IgG (Abcam).
Claeys Bouuaert C., Tischfield S.E., Pu S., Mimitou E.P., Arias-Palomo E., Berger J.M, & Keeney S. (2021). Structural and functional characterization of the Spo11 core complex. Nature structural & molecular biology, 28(1), 92-102.
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3

Western Blot Analysis of Protein Extracts

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Denaturing whole-cell extracts were prepared in10% trichloroacetic acid by agitation with glass beads. Proteins were solubilized in Laemmli sample buffer, separated by SDS-PAGE and analyzed by western blotting. Antibodies were goat polyclonal anti-LexA (1:2000, Santa Cruz), rat polyclonal anti-α-tubulin (1:5000, Bio-rad), mouse monoclonal anti-Flag M2 (1:2000, Sigma) HRP-conjugated mouse monoclonal anti-Flag M2 (1:2000, Sigma), mouse monoclonal anti-MBP (1:2000, NEB), rabbit polyclonal anti-Spo11 (1:1000, this laboratory). Secondary antibodies were used at 1:5000 dilution: IRDye 800CW goat anti-mouse IgG (LI-COR), HRP-conjugated goat anti-mouse IgG (Bio-Rad), HRP-conjugated goat anti-rabbit IgG (Bio-Rad), HRP-conjugated donkey anti-goat IgG (Santa Cruz), HRP-conjugated donkey anti-rat IgG (Abcam).
Claeys Bouuaert C., Tischfield S.E., Pu S., Mimitou E.P., Arias-Palomo E., Berger J.M, & Keeney S. (2021). Structural and functional characterization of the Spo11 core complex. Nature structural & molecular biology, 28(1), 92-102.
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4

Detecting Protein Interactions by Western Blot

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Denaturing whole-cell extracts were prepared in 10% trichloroacetic acid with agitation in the presence of glass beads. Precipitated proteins were solubilized in Laemmli sample buffer and appropriate amounts of protein were separated by SDS-PAGE and analyzed by western blotting. Antibodies were mouse monoclonal anti-myc (1/2000, Abcam), rabbit polyclonal anti-Kar2 (y-115) (1/2000, Santa Cruz), HRP-conjugated mouse monoclonal anti-Flag M2 (1:2000, Sigma), mouse monoclonal anti-MBP (1:2000, NEB). Secondary antibodies were used at 1/5000: IRDye 800CW goat anti-mouse IgG, IRDye 680 goat anti-rabbit IgG. Western blots were revealed using the Li-COR Bioscience Odyssey infrared imaging system.
Claeys Bouuaert C., Pu S., Wang J., Oger C., Daccache D., Xie W., Patel D.J, & Keeney S. (2021). DNA-driven condensation assembles the meiotic DNA break machinery. Nature, 592(7852), 144-149.
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5

Detecting Protein Interactions by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Denaturing whole-cell extracts were prepared in 10% trichloroacetic acid with agitation in the presence of glass beads. Precipitated proteins were solubilized in Laemmli sample buffer and appropriate amounts of protein were separated by SDS-PAGE and analyzed by western blotting. Antibodies were mouse monoclonal anti-myc (1/2000, Abcam), rabbit polyclonal anti-Kar2 (y-115) (1/2000, Santa Cruz), HRP-conjugated mouse monoclonal anti-Flag M2 (1:2000, Sigma), mouse monoclonal anti-MBP (1:2000, NEB). Secondary antibodies were used at 1/5000: IRDye 800CW goat anti-mouse IgG, IRDye 680 goat anti-rabbit IgG. Western blots were revealed using the Li-COR Bioscience Odyssey infrared imaging system.
Claeys Bouuaert C., Pu S., Wang J., Oger C., Daccache D., Xie W., Patel D.J, & Keeney S. (2021). DNA-driven condensation assembles the meiotic DNA break machinery. Nature, 592(7852), 144-149.
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6

Antibody Characterization and Protein Detection

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Custom-made, affinity-purified antibodies generated against two short amino acid stretches of rat Jacob (285-299 and 400-314, Jb150) have been described previously [14, 21, 22] . The following commercial primary antibodies were used in the present study:
mouse monoclonal anti-MAP2 (Sigma-Aldrich, M4403, mouse monoclonal anti-LaminB1
(ProteinTeck, 3C10G12), rabbit polyclonal anti-CRM1 (ProteinTeck), mouse monoclonal anti-MBP (NEB), mouse monoclonal anti-GFP (BioLegend, B34), and rabbit polyclonal anti-tRFP (EVROGEN). For imaging and WB analysis, the following secondary antibodies were used: anti-rabbit, anti-mouse secondary antibodies conjugated with Alexa Fluor 488, 561 or 647 (Life Technologies), peroxidase-conjugated anti-mouse IgG (Dianova)
and anti-rabbit IgG (Dianova).
Samer S., Raman R., Laube G., Kreutz M.R., & Karpova A. (2020). The nuclear lamina is a hub for the nuclear function of Jacob.
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