Apochromat water immersion objectives

All analyses on human tissue were performed as we previously described^{61 (link)}. Heat-induced antigen retrieval was performed following Dako's protocol. For immunofluorescence analysis, we used the following primary antibodies: for pericyte coverage - polyclonal goat anti-human PDGFRβ (R&D systems, AF385; 1:100), for fibrinogen and fibrin extravascular deposits - polyclonal rabbit anti-human fibrinogen (Dako, A0080; 1:500), and species-specific fluorochrome-conjugated secondary antibodies were incubated (see table below) for 1 h at room temperature. Blood vessel endothelial profiles were stained by Dylight 488-conjugated L. esculentum lectin (Vector Labs, DL-1174; 1:200) for 1 h at room temperature. All slices were scanned using Zeiss 510 confocal microscope with Zeiss Apochromat water immersion objectives (Carl Zeiss MicroImaging Inc., Thornwood, NY, USA).

Heat-induced antigen retrieval was performed following Dako’s protocol. For immunohistochemistry analysis of PICALM or Aβ, ImmPRESS™ Polymer-Based Immunohistochemistry Reagents (Vector Laboratories) were used for visualization. For immunofluorescence analysis, species-specific fluorochrome-conjugated secondary antibodies were incubated for 1 h at room temperature, and blood vessels were stained by Dylight 488-conjugated L. esculentum lectin for 1 h at room temperature. All slices were scanned using Zeiss 510 confocal microscope with Zeiss Apochromat water immersion objectives (Carl Zeiss MicroImaging Inc., Thornwood, NY, USA).