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8 br adpr

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Sourced in Germany

8-Br-ADPR is a chemical compound that functions as an adenosine diphosphate ribose (ADPR) analog. It can be utilized in various biochemical and cell-based applications, though its specific intended uses are not provided.

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Lab products found in correlation

Xestospongin c, by Santa Cruz Biotechnology (3 mentions) Ara 2 f nad, by Biolog (2 mentions) Rottlerin, by Bio-Techne (1 mentions) Go6976, by Bio-Techne (1 mentions) Skf96365, by Bio-Techne (1 mentions) Trans ned19, by Bio-Techne (1 mentions) Thapsigargin, by Bio-Techne (1 mentions) Gsk2578215a, by Bio-Techne (1 mentions) Egta am, by AnaSpec (1 mentions) Anti mouse cd38, by BioLegend (1 mentions) Bapta am, by R&D Systems (1 mentions) Naadp am, by AAT Bioquest (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Chir99021, by Bio-Techne (1 mentions) Dorsomorphin, by Bio-Techne (1 mentions) Gsk2606414, by Bio-Techne (1 mentions) Rtk2758, by BioLegend (1 mentions) Lps e coli serotype r515, by Enzo Life Sciences (1 mentions) Phospho foxo1, by Cell Signaling Technology (1 mentions) Ned 19, by Enzo Life Sciences (1 mentions)

4 protocols using 8 br adpr

1

Inhibitors of Sarcoplasmic Reticulum Calcium Release

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Xestospongin C was obtained from Santa Cruz Biotechnology. Trans-Ned19, GF10923X, Go6976, rottlerin, and SKF96365 were obtained from Tocris Bioscience. 8-Br-ADPR and ara-2’-F-NAD were obtained from Biolog Life Science Institute. All other reagents were obtained from Sigma-Aldrich.
Rah S.Y., Joe Y., Park J., Ryter S.W., Park C., Chung H.T, & Kim U.H. (2023). CD38/ADP-ribose/TRPM2-mediated nuclear Ca2+ signaling is essential for hepatic gluconeogenesis in fasting and diabetes. Experimental & Molecular Medicine, 55(7), 1492-1505.
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2

Reagents for Immune Cell Stimulation

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The following antibodies were used for cell stimulation: purified anti-mouse CD38 (Clone 90; BioLegend, 102702), purified rat IgG2a, κ isotype control (Clone RTK2758; BioLegend, 400565). In certain experiments, the following reagents/inhibitors were used at the indicated concentration in cell culture: LPS (E. Coli Serotype R515; Enzo Life Sciences, ALX-581-007-L001), NAADP-AM (3 μM; AAT Bioquest, 21000), GSK2578215A (LRRK2 inhibitor, 1 µM; Tocris Bioscience, 4629), GPN (200 µM; Santa Cruz Biotechnology, sc-252858), EDTA (2 mM; Sigma-Aldrich, E6758), thapsigargin (500 nM; Tocris Bioscience, 1138), 8-Br-cADPR (100 µM; BIOLOG Life Science Institute, B065), 8-Br-ADPR (100 µM; BIOLOG Life Science Institute, B051), Ned-19 (100 µM; Cayman Chemical, 17527), cyclosporine (10 µM; R&D Systems, 1101/100), FK506 (5 nM; Eton Bioscience Inc, 1100060052), CHIR 99021 (GSK3B inhibitor, 5 µM; Tocris Bioscience, 4423), BAPTA-AM (10 µM; R&D Systems, 2787), EGTA-AM (10 µM; AnaSpec Inc, AS-84100), GSK2606414 (EIF2AK3/PERK inhibitor, 10 µM; Tocris Bioscience, 5107), and dorsomorphin (5 µM; Tocris Bioscience, 3093).
Nabar N.R., Heijjer C.N., Shi C.S., Hwang I.Y., Ganesan S., Karlsson M.C, & Kehrl J.H. (2021). LRRK2 is required for CD38-mediated NAADP-Ca2+ signaling and the downstream activation of TFEB (transcription factor EB) in immune cells. Autophagy, 18(1), 204-222.
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3

Antibody Acquisition and Reagent Sourcing

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Antibodies were obtained as follows: granzyme B pAb from Cell Signaling (Danvers, MA) and perforin pAb from Santa Cruz Biotechnology (Santa Cruz, CA); anti-perforin mAb, DyLight 488 anti-perforin mAb, and DyLight 550 anti-TRPM2 pAb from Novus Biologicals (Littleton, CO). Human recombinant IL-2 was from Chiron BV (Amsterdam, Netherlands) and Xestospongin C was from Santa Cruz Biotechnology (Santa Cruz, CA). 8-Br-ADPR, ara-2′-F-NAD, and N-(p-amylcinnamoyl) anthranilic acid (ACA) were from Biolog Life Science Institute (Bremen, Germany). All other reagents were obtained from Sigma-Aldrich (St. Louis, MO).
Rah S.Y., Kwak J.Y., Chung Y.J, & Kim U.H. (2015). ADP-ribose/TRPM2-mediated Ca2+ signaling is essential for cytolytic degranulation and antitumor activity of natural killer cells. Scientific Reports, 5, 9482.
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4

Antibodies and Reagents for CREB Signaling

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Antibodies were obtained as follows: CREB, phospho-CREB, FoxO1, and phospho-FoxO1 from Cell Signaling (Danvers, MA), CRTC2 from R&D Systems and Actin from Millipore. 8-Br-ADPR from Biolog Life Science Institute (Bremen, Germany) and Ned19 from Enzo Life Sciences (NY, USA), and all other reagents were obtained from Sigma-Aldrich (St. Louis, MO). Xestospongin C was from Santa cruz biotechnology.
Rah S.Y, & Kim U.H. (2015). CD38-mediated Ca2+ signaling contributes to glucagon-induced hepatic gluconeogenesis. Scientific Reports, 5, 10741.
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