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Gly pro amido 4 methylcoumarin hydrobromide gly pro amc

Manufactured by Merck Group
Sourced in United States

Gly-Pro-amido-4-methylcoumarin hydrobromide (Gly-Pro-AMC) is a fluorogenic substrate used for the quantitative determination of dipeptidyl peptidase IV (DPP-IV) activity. It is a synthetic compound that emits fluorescence upon enzymatic cleavage.

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2 protocols using gly pro amido 4 methylcoumarin hydrobromide gly pro amc

1

Sitagliptin Modulates Peptide Cellular Uptake

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Dulbecco’s modified Eagle medium (DMEM) was obtained from GIBCO (Thermo Fisher Scientific, Waltham, MA USA). Fetal bovine serum (FBS) was from Hyclone Laboratories (Logan, UT, USA). Stable L-glutamine, 1% non-essential amino acids, penicillin/streptomycin, and PBS were from Euroclone (Milan, Italy). Sitagliptin and Gly-Pro-amido-4-methylcoumarin hydrobromide (Gly-Pro-AMC) were from Sigma-Aldrich (St. Louis, MO, USA). Polycarbonate filters, 12 mm in diameter, 0.4 m in pore diameter, were from Transwell Corning Inc. (Lowell, MA, USA). Peptides Lup1 (LTFPGSAED) and Soy1 (IAVPTGVA) were synthetized by the company PRIMM (Milan, Italy) at >95% purity. Human serum was freshly collected from a healthy female volunteer.
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2

Casein Hydrolysate Bioactive Peptides

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Dulbecco’s modified Eagle’s medium (DMEM), L-glutamine, fetal bovine serum (FBS), phosphate buffered saline (PBS), penicillin/streptomycin and 96-well plates were purchased from Euroclone (Milan, Italy). Acetonitrile (ACN), formic acid, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), sitagliptin, Gly-Pro-amido-4-methylcoumarin hydrobromide (Gly-Pro-AMC) and ACE from porcine kidney were from Sigma-Aldrich (St. Louis, MO, United States). ACE1 Activity Assay Kits come from Biovision (Milpitas Blvd., Milpitias, CA, United States). The DPP-IV inhibitor screening assay kit was bought from Cayman Chemical (Michigan, United States). Caco-2 cells were obtained from INSERM (Paris, France). Casein hydrolysates (CH), which is spray dried samples from the production process, was supplied by A. Costantino S.R.L. (Italy). (Casein hydrolysate sport/health batch 218F0004, 100 g). Casein hydrolysates (CH), which is spray dried samples from the production process, was supplied by A. Costantino S.R.L. (Italy). (Casein hydrolysate sport/health batch 218F0004, 100 g).
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