Gly pro amido 4 methylcoumarin hydrobromide gly pro amc

Dulbecco’s modified Eagle medium (DMEM) was obtained from GIBCO (Thermo Fisher Scientific, Waltham, MA USA). Fetal bovine serum (FBS) was from Hyclone Laboratories (Logan, UT, USA). Stable L-glutamine, 1% non-essential amino acids, penicillin/streptomycin, and PBS were from Euroclone (Milan, Italy). Sitagliptin and Gly-Pro-amido-4-methylcoumarin hydrobromide (Gly-Pro-AMC) were from Sigma-Aldrich (St. Louis, MO, USA). Polycarbonate filters, 12 mm in diameter, 0.4 m in pore diameter, were from Transwell Corning Inc. (Lowell, MA, USA). Peptides Lup1 (LTFPGSAED) and Soy1 (IAVPTGVA) were synthetized by the company PRIMM (Milan, Italy) at >95% purity. Human serum was freshly collected from a healthy female volunteer.

Dulbecco’s modified Eagle’s medium (DMEM), L-glutamine, fetal bovine serum (FBS), phosphate buffered saline (PBS), penicillin/streptomycin and 96-well plates were purchased from Euroclone (Milan, Italy). Acetonitrile (ACN), formic acid, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), sitagliptin, Gly-Pro-amido-4-methylcoumarin hydrobromide (Gly-Pro-AMC) and ACE from porcine kidney were from Sigma-Aldrich (St. Louis, MO, United States). ACE1 Activity Assay Kits come from Biovision (Milpitas Blvd., Milpitias, CA, United States). The DPP-IV inhibitor screening assay kit was bought from Cayman Chemical (Michigan, United States). Caco-2 cells were obtained from INSERM (Paris, France). Casein hydrolysates (CH), which is spray dried samples from the production process, was supplied by A. Costantino S.R.L. (Italy). (Casein hydrolysate sport/health batch 218F0004, 100 g). Casein hydrolysates (CH), which is spray dried samples from the production process, was supplied by A. Costantino S.R.L. (Italy). (Casein hydrolysate sport/health batch 218F0004, 100 g).