Mice were maintained in the Centenary Institute animal facility under specific pathogen free conditions and exposed to a 12 h light-dark cycle. C57BL/6J and PTPRCA mice were purchased from either Animal Resource Centre (Perth, WA, Australia) or Australian BioResources (Moss Vale, NSW, Australia). Mice were housed 4–6 mice per cage with ad libitum access to rodent chow (Specialty Feeds, Perth, Western Australia) and water and entered experiments more than one week after any transport. All the mice used for chimera experiments were at least 8 weeks old before irradiation.
The DPP9S729A mouse strain has been described15 (link). All embryos required for fetal liver cells were obtained from pregnant females where pregnancy resulted from timed mating of DPP9 heterozygous intercrosses. For mating, single females were placed in the male home cage for 12 h then checked for the presence of a vaginal plug as an indicator of mating prior to separation from the male. The time of separation of female and male was designated ED 0.5. Females were weighed at ED 0.5 and weighed daily from ED 4.5 until ED 13. Pregnancy was suggested by weight gain greater than non-pregnant between ED 7.5 and ED 13.539 (link).
The DPP9S729A mouse strain has been described15 (link). All embryos required for fetal liver cells were obtained from pregnant females where pregnancy resulted from timed mating of DPP9 heterozygous intercrosses. For mating, single females were placed in the male home cage for 12 h then checked for the presence of a vaginal plug as an indicator of mating prior to separation from the male. The time of separation of female and male was designated ED 0.5. Females were weighed at ED 0.5 and weighed daily from ED 4.5 until ED 13. Pregnancy was suggested by weight gain greater than non-pregnant between ED 7.5 and ED 13.539 (link).