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Fitc conjugated rabbit anti guinea pig or anti pig igg

Manufactured by Merck Group

FITC conjugated rabbit anti-guinea pig or anti-pig IgG is a laboratory reagent used for the detection and quantification of guinea pig or pig immunoglobulin G (IgG) in various immunoassays and research applications. The product consists of polyclonal rabbit antibodies specifically directed against guinea pig or pig IgG, which are conjugated with the fluorescent dye fluorescein isothiocyanate (FITC).

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2 protocols using fitc conjugated rabbit anti guinea pig or anti pig igg

1

Visualizing Rab7-Mediated Endocytic Pathways

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GCDCA and CDCA were purchased from Sigma-Aldrich (St. Louis, MO) and resuspended in distilled water or dimethyl sulfoxide (DMSO), respectively, at 100 mM. Hyperimmune guinea pig sera raised against recombinant PEC 2AB, polymerase (POL) and VPg and hyperimmune swine sera raised against PEC virus-like particles (Chang et al., 2005 (link)) were used in this study. Rabbit polyclonal anti-Rab7 serum was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Secondary antibodies including FITC conjugated rabbit anti-guinea pig or anti-pig IgG and PerCP-Cy5.5 labelled goat anti-rabbit IgG were purchased from commercial sources including Sigma-Aldrich and Santa Cruz Biotechnology. Sytox orange was obtained from Molecular Probes (Bedford, MA). Chloroquine, GW4064, and oleanolic acid were also purchased from Sigma-Aldrich. Other basic chemicals for confocal microscopy and other studies were purchased from various sources including Sigma-Aldrich.
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2

Investigating Viral Protein Interactions Using Immunoassays

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GCDCA and CDCA were purchased from Sigma-Aldrich (St. Louis, MO) and resuspended in distilled water or dimethyl sulfoxide (DMSO), respectively, at 100 mM. Hyperimmune guinea pig sera raised against recombinant PEC 2AB, polymerase (POL) and VPg and hyperimmune swine sera raised against PEC virus-like particles (Chang et al., 2005 (link)) were used in this study. Rabbit polyclonal anti-Rab7 serum was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Secondary antibodies including FITC conjugated rabbit anti-guinea pig or anti-pig IgG and PerCP-Cy5.5 labelled goat anti-rabbit IgG were purchased from commercial sources including Sigma-Aldrich and Santa Cruz Biotechnology. Sytox orange was obtained from Molecular Probes (Bedford, MA). Chloroquine, GW4064, and oleanolic acid were also purchased from Sigma-Aldrich. Other basic chemicals for confocal microscopy and other studies were purchased from various sources including Sigma-Aldrich.
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