Fitc conjugated mouse anti human cd41a

Salidroside was purchased from MedChemExpress (Monmouth Junction, NJ, USA) with a purity ≥ 98%. Collagen-related peptide (CRP) was prepared as previously described [56 (link)]. Thrombin (≥ 10 NIH units/vial) were from Chrono-log Corporation (Havertown, PA, USA). FITC-conjugated mouse anti-human CD41a and PAC-1 antibody were from BD Biosciences (San Jose, CA, USA) and BECTON DICKINSON (San Jose, CA, USA) respectively. PE-conjugated anti-human/mouse CD62p (P-Selectin) and anti-human Glycoprotein VI purified antibody were purchased from eBioscience (San Diego, CA, USA). FITC-conjugated anti-CD42b antibody was from Abcam (Cambridge, MA, USA). FITC-conjugated goat anti-mouse IgG was purchased from ZSGB-BIO (Beijing, China). β-actin antibody and anti-rabbit IgG (HRP-linked) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). SB216763 (GSK-3β inhibitor) was purchased from ApexBio Technology.

Matrine was purchased from MedChemExpress (Monmouth Junction, NJ, United States) with a purity ≥98% and dissolved in saline. Collagen-related peptide (CRP) was prepared as previously described (Arthur et al., 2012 (link)). Collagen and thrombin (≥10 NIH units/vial) were from Chrono-log Corporation (Havertown, PA, United States). Fibrinogen and BSA (bovine serum albumin) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Mepacrine (also known as Quinacrine) was from APExBIO (Boston, MA, United States). FITC-conjugated mouse anti-human CD41a and PAC-1 antibody were from BD Biosciences (San Jose, CA, United States). PE-labelled anti-human/mouse CD62p (P-Selectin) and anti-human Glycoprotein VI antibody were purchased from eBioscience (San Diego, CA, United States). Alexa Fluor-546-labelled phalloidin was purchased from Thermo Fisher Scientific (Waltham, MA, United States). FITC-conjugated goat anti-mouse IgG was purchased from ZSGB-BIO (Beijing, China). β-actin antibody and HRP-conjugated anti-rabbit IgG were purchased from Cell Signaling Technology (Danvers, MA, United States).

Platycodin D (PD) was purchased from Fluka (Steinheim, Germany) with a purity ≥ 99%. Collagen, adenosine diphosphate (ADP), arachidonic acid and epinephrine were from Helena laboratories (Beaumont, Texas, USA). Thrombin was purchased from Sigma-Aldrich (St. Louis, MO, USA). FITC-conjugated mouse anti-human CD41a and PAC-1 antibody were from BD Biosciences (San Jose, CA, USA) and Becton–Dickinson (San Jose, CA, USA) respectively. PE-conjugated anti-human/mouse CD62p (P-Selectin) and anti-human Glycoprotein VI purified antibody were purchased from eBioscience (San Diego, CA, USA). FITC-conjugated anti-CD42b antibody was from Abcam (Cambridge, MA, USA). FITC-conjugated goat anti-mouse IgG was purchased from ZSGB-BIO (Beijing, China). β-actin antibody and anti-rabbit IgG (HRP-linked) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA).