Inb 400

Manufactured by Memmert

Sourced in Germany

The INB 400 is a universal incubator from Memmert. It is designed for the temperature-controlled incubation of samples in laboratories.

Automatically generated - may contain errors

Lab products found in correlation

Allegra x 22r, by Beckman Coulter (1 mentions) Whitley a35 anaerobic workstation, by Don Whitley Scientific (1 mentions) Varioskan lux multimode microplate reader, by Thermo Fisher Scientific (1 mentions)

5 protocols using inb 400

Evaluating Graft Material Ionic Release

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A sample of 0.5 g from each graft material was added to 10 mL of PBS and maintained in a drying oven (INB 400, Memmert, Germany) at 37°C. The samples were collected on days 3, 7, and 14. On day 3, PBS containing 0.5 g graft materials was centrifuged (AllegraTM, X-22R, Beckman Coulter, CA, USA) at 1,000 rpm for 5 min and 10 mL of supernatant was obtained. The unchanged lot of 10 mL PBS was added to the same 0.5 g of graft materials, then stored for another four days. After 7 days, PBS containing 0.5 g graft materials was centrifuged at 1,000 rpm for 5 min and 10 mL of supernatant was obtained. The same procedure was repeated, and the sample was stored for another 7 days.
At each time interval, the same lot PBS was used as the blanks for measuring amounts of calcium (Ca) and phosphorus (P) ions presence and compared with the amounts of Ca and P ions from PBS containing 0.5 g graft materials. The representative amounts of Ca and P ions released in the supernatant were once measured at days 3, 7, and 14 by inductively coupled plasma-mass spectroscopy (ICP-MS) (7500ce models, Agilent) and ion chromatography (IC) (ICS-3000 models, DIONEX), respectively.

Khanijou M., Zhang R., Boonsiriseth K., Srisatjaluk R.L., Suphangul S., Pairuchvej V., Wongsirichat N, & Seriwatanachai D. (2021). Physicochemical and osteogenic properties of chairside processed tooth derived bone substitute and bone graft materials. Dental materials journal, 40(1).

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Counting Bacterial Colony Forming Units

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Different agar plates were prepared for the characterization of CFU samples. Table 2 provides the agar media used for the different bacteria species. The plating was performed under aseptic conditions. Samples from the time points 0, 12 and 24 h were plated out. The cryo samples were diluted with sterilized peptone water according to the following dilution series. The dilution series was performed in steps of ten from 1 to 1 million. For each dilution, 20 µL was plated on each agar medium. The inoculated agar plates were incubated for two days under aerobic conditions (INB 400, Memmert GmbH, Schwabach, Germany) at 33 °C or under anaerobic conditions (Whitley A35 Anaerobic Workstation, Don Whitley Scientific, Bingley, UK) at 37 °C for five days. The CFU were determined by counting. The plate sections with the highest concentration and greatest bacterial growth, ranging from 3 to 90 colonies, were used for counting. CFU/mL was calculated using the following Equation (1). A sample volume of 20 µL was used (‘50’) and the sample was mixed with glycerol in a ratio of 1:2 (‘2’).

\frac{C F U}{m L} = C F U \times d i l u t i o n f a c t o r \times 50 \times 2

Seradj D.S., Beeck R., Haase A., Krause J., Schick P, & Weitschies W. (2023). Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol3. Pharmaceuticals, 16(8), 1128.

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Isolation and Purification of Yeast Strains

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Two methods were tested for the isolation of yeast strains. The method of successive dilutions on yeast peptone dextrose Agar (YPDA) medium [19] (link) included spreading with a glass spreader, alongside an incubation period between three to seven days at 28 °C (Memmert INB 400, Germany). After macroscopic and microscopic observation, purification was carried out by streaking on yeast extract malt extract agar (YMA) medium and incubated until pure isolates were obtained. The pure strains were stored at 4 °C in sabouraud dextrose agar (SDA) supplemented with chloramphenicol and in yeast extract malt extract (YM) medium supplemented with 30% glycerol at −20 °C.
The enrichment method was also used for isolation. This is a procedure in which 2 mL of thermal water inoculum as the source of yeast strains was incubated in 50 mL YM broth using 250 mL Erlenmeyer flasks at 30 °C on a rotary shaker (Stuart SI500, UK) at 150 rpm for 24 hours. After incubation, the spread was performed on YM agar, YGA, and SDA, and incubated at 28 °C for three to seven days [10] (link).

Chaib I., Dakhmouche-Djekrif S., Bennamoun L, & Nouadri T. (2024). Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast Geotrichum candidum PO27. AIMS Microbiology, 10(1), 83-106.

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Moisture Content Determination in Dried Samples

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The fresh samples (50 g) were dried in a conventional oven (INB 400, Memmert) at 105°C for 24 h. Moisture content (based on fresh weight) was obtained using Equation (1):

M_{w} = \frac{(W_{w} - W_{d})}{W_{w}} \times 100,

M_w: moisture weight based on fresh weight (%), W_w: fresh weight (g), and W_d: dry weight (g).

Zamani S., Bakhshi D., Sahraroo A, & Ebadi M. (2023). Improvement of phytochemical and quality characteristics of Dracocephalum kotschyi by drying methods. Food Science & Nutrition, 11(7), 4246-4262.

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Quantification of Colon and Lymph Node ROS

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The ROS concentration in colon and mesenteric lymph nodes was quantified using the method described by [24 (link)]. Frozen colon and mesenteric lymph nodes samples (100 mg) were homogenized (1:10 w/v) in 1 mL of ice-cold Tris-HCl buffer (40 mM, pH = 7.4), using UltraTurrax homogenizer (IKAWerke GmbH &Co. KG, Staufen, Germany). 100 μl of tissue homogenates were mixed with 1 mL of Tris-HCl buffer and 5 μl of 10 μM DFCA DA (2`,7`-dichlorofluorescein diacetate, Sigma-Aldrich, St. Louis, MO, USA). Another pool of 100 μl of tissue homogenate were mixed with 1 mL of Tris-HCl buffer and used as control of tissue autofluorescence. The samples were incubated for 40 minutes at 37 ˚C, with agitation (Precision Incubators INB 400, Memmert GmbH, Schwabach, Germany). After incubation, 200 μl of each sample was transferred in 96-well optical-bottom plates (Nunc, Thermo Fischer Scientific, Waltham, MA, USA), in duplicate. The ROS analysis was performed in triplicate for each sample. The fluorescence intensity of the samples was measured using Varioskan™ LUX multimode microplate reader (λ_excitation = 485 nm and λ_emission = 525 nm) (Thermo Fisher Scientific, Waltham, MA, USA).

Pistol G.C., Marin D.E., Bulgaru V.C., Anghel A.C., Sărăcilă M., Vlassa M., Filip M, & Taranu I. (2023). Grape seed meal by-product is able to counteract oxidative stress induced by lipopolysaccharide and dextran sulphate in IPEC cells and piglets after weaning. PLOS ONE, 18(4), e0283607.

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