Peptone

Manufactured by Loba Chemie

Sourced in India

Peptone is a protease-digested form of protein, typically derived from animal sources such as casein or meat. It is a complex mixture of amino acids, peptides, and other nitrogenous compounds. Peptone is commonly used as a nutrient source in microbiological culture media, providing essential nutrients for the growth and proliferation of various microorganisms.

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Lab products found in correlation

2 protocols using peptone

Natural Antimicrobial Polymer Formulations

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Sodium alginate with a medium viscosity and high mannuronic acid without inulin were purchased from Hi-media (Mumbai). They were prepared in distilled water and autoclaved at 121 °C for 15 min. The low-molecular-weight chitosan (deacetylated chitin, Hi-Media, Laboratories Mumbai), was prepared in distilled water and pure hydrochloric acid (Loba Chemie, Pvt Ltd.—Mumbai, India). Xanthan gum, gum acacia, and carrageenan were purchased from Loba Chemie, Pvt Ltd.—Mumbai, India. Starch, sodium caseinate, pepsin, trypsin, calcium chloride, sodium chloride, sodium bicarbonate, trisodium citrate, sodium hydroxide, potassium chloride, phosphate buffer saline (pH 7.2), and hydrochloric acid were purchased from Hi-media Laboratories (Mumbai, India). Glacial acetic acid with molar mass of 60.05 g mol⁻¹ was bought from Merck (Darmstadt, Germany). Pancreatin, peptone, and bile salt were obtained from Loba Chemie, Pvt Ltd. (Mumbai, India). The De Man Rogosa and Sharpes (MRS) broth and MRS agar used in this work were purchased from Hi-media Laboratories (Mumbai). Indicator microorganisms used for the antimicrobial activity; i.e., Escherichia coli (MTCC No-432), Staphylococcus aureus (MTCC No-96), and Bacillus cereus (MTCC No-430), were procured from the Institute of Microbial Technology (IMTECH; Chandigarh, India).

Oberoi K., Tolun A., Altintas Z, & Sharma S. (2021). Effect of Alginate-Microencapsulated Hydrogels on the Survival of Lactobacillus rhamnosus under Simulated Gastrointestinal Conditions. Foods, 10(9), 1999.

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Enzymatic Hydrolysis of Defatted Soybean Meal

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Defatted soybean meal (48% protein, 28.6% total sugar, 9.7% reducing sugar, 6% lipid, 9.0% ash, and 8.4% moisture) was obtained from Food Technology Research Institute, Agric. Res. Center, Giza, Egypt. Amino acids; threonine and lysine, authentic compounds, and standard n-paraffin (C₈-C₂₂) were purchased from Sigma Aldrich Chemical Co. (St. Louis, MO, USA). Flavourzyme (from Aspergillus oryzae) and Alcalase (from Bacillus Licheniformis) were obtained from Novo Nordisk A/S (NOVO ALLE, DK - 2880, Bagsvaerd, Denmark). Glucose, agar and H₂SO₄ were purchased from Merck Company, Germany. Peptone, yeast extracts and di-ammonium phosphate was purchased from Loba Chemie, Bombay, India. DNS was purchased from Sigma Aldrich Chemical Co. Filter papers (Whatman No. 1, 15 cm diameter) Whatman International Ltd Maidstone, England.

Fadel H.H., Lotfy S.N., Asker M.M., Mahmoud M.G, & Al-Okbi S.Y. (2018). Nutty-like flavor production by Corynbacterium glutamicum 1220T from enzymatic soybean hydrolysate. Effect of encapsulation and storage on the nutty flavoring quality. Journal of Advanced Research, 10, 31-38.

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