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Nadph tetrasodium salt

Manufactured by Enzo Life Sciences
Sourced in United States

NADPH tetrasodium salt is a chemical compound that serves as a cofactor in various enzymatic reactions. It is a salt form of nicotinamide adenine dinucleotide phosphate (NADPH), which is an essential molecule for cellular metabolism and energy production.

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2 protocols using nadph tetrasodium salt

1

Enzymatic Assay for Screening 3-Nitro-2-Phenyl-2H-Chromene Analogues

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Adenosine 5′-triphosphate disodium salt hydrate (≥99%), chlorophenol red-β-d-galactopyranoside (CPRG), β-nicotinamide adenine dinucleotide phosphate hydrate (NADP+), magnesium chloride hexahydrate (≥99%), d-(+)-glucose (≥99%), dimethyl sulfoxide (ACS reagent (≥99.9%)), d-glucose-6-phosphate sodium salt, N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES, ≥99.5%), imidazole (96.0%), Terrific broth modified, and triethanolamine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from CellGro Technologies, LLC (Lincoln, NE, USA). Leuconostoc mesenteroides glucose-6-phosphate dehydrogenase (LmG6PDH) was purchased from Worthington Biochemical Corporation (Lakewood, NJ, USA). NADPH tetrasodium salt (≥95%) was purchased from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). For the 3-nitro-2-phenyl-2H-chromene analogues, compounds 17 were purchased from TimTec, LLC (Tampa, FL, USA), while compounds 813 were purchased from ChemDiv, Inc. (San Diego, CA, USA). Ethylenediaminetetraacetic acid (EDTA) tetrasodium salt hydrate (98%), Luria-Bertani (LB) broth, lysozyme (type VI), protease inhibitor tablets (EDTA-free), glycerol, and all other chemicals were purchased from Fisher Scientific (Hampton, NH, USA).
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2

In Vitro Iohexol Elimination Assay

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Sterile sodium chloride (0.9% NaCl) and phosphate-buffered saline (PBS) were purchased from Baxter Healthcare Corporation (Deerfield, IL). Iohexol powder, paclitaxel, and gemcitabine were purchased from Toronto Research Chemicals Inc (Toronto, ON, Canada). Iohexol dosing solution was manufactured by GE Healthcare, (Shanghai, China) and obtained from the Hillman Cancer Research Pharmacy. All in vitro drug elimination incubations with iohexol were performed at 300 μg/mL which is at the upper range of the reported plasma concentrations [23 (link)]. [D5]-iohexol (IS) was purchased from ALSACHIM (Graffenstaden, France). Carboplatin for dosing was manufactured by Ben Venue Labs Inc (Bedford, OH) and obtained from the Hillman Cancer Research Pharmacy. NADPH tetrasodium salt was purchased from Enzo Life Sciences (Farmingdale, NY). Carboplatin powder, MgCl2, alamethicin, UDPGA sodium salt, phenacetin, dextromethorphan and midazolam methanolic solution was obtained from Sigma (St Louis, MO). [D3]-dextrorphan and [D4]-1-hydroxy midazolam were purchased from Sigma (St. Louis MO). 1-hydroxy midazolam, 4-hydroxy diclofenac, 4-hydroxy mephenytoin, dextrorphan tartrate, s-mephenytoin, SN-38, trifluoperazine, mycophenolic acid and naloxone were purchased from Cayman chemicals (Ann Arbor, MI).
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