Various chemical reagents were sourced from suppliers across different regions. Ferric chloride and sodium nitrite were acquired from Ajax Finechem (New South Wales, Australia). Gallic acid and CIP hydrochloride were purchased from Bio Basic Inc. in Markham, ON, Canada. Epigallocatechin gallate and quercetin were procured from Sigma–Aldrich (Steinheim, Germany). Copper sulfate pentahydrate and hydrochloric acid were purchased from QRëC (Auckland, New Zealand). Chloroform and acetic acid were from JT Baker (Phillipsburg, NJ, USA). Folin–Ciocalteu reagent was purchased from Carlo Erba (Barcelona Spain). Ammonium chloride, magnesium chloride hexahydrate, manganese sulfate, phenol detached crystals, and sodium hypochlorite were acquired from Loba Chemie (Mumbai, India). Aluminum chloride hexahydrate, bromophenol blue, sodium acetate, and calcium carbonate were acquired from KemAu (Sydney, Australia). Vanillin was purchased from Merck (Darmstadt, Germany). We obtained sodium hydroxide and sodium chloride from RCI Labscan located in Thailand. Other chemicals and solvents were of the highest grade available.
Gallic acid
Manufactured by Bio Basic
Sourced in Canada
Gallic acid is a natural phenolic compound that is commonly used in a variety of laboratory applications. It is a crystalline solid that is soluble in water and organic solvents. Gallic acid is a versatile molecule that can be utilized for various analytical and research purposes, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Quercetin, by Merck Group (3 mentions)
Ferric chloride, by Thermo Fisher Scientific (2 mentions)
Epigallocatechin gallate (egcg), by Merck Group (2 mentions)
Sodium nitrite, by Thermo Fisher Scientific (2 mentions)
Sodium hydroxide (naoh), by RCI Labscan (2 mentions)
Vanillin, by Merck Group (2 mentions)
Cip hydrochloride, by Bio Basic (2 mentions)
Acetic acid, by Avantor (1 mentions)
Chloroform, by Avantor (1 mentions)
Ammonium chloride, by Loba Chemie (1 mentions)
Magnesium chloride hexahydrate, by Loba Chemie (1 mentions)
Manganese sulfate, by Loba Chemie (1 mentions)
Sodium hypochlorite, by Loba Chemie (1 mentions)
Folin ciocalteu reagent, by Carlo Erba (1 mentions)
Absolute ethanol, by Avantor (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Andrographolide, by Merck Group (1 mentions)
Clindamycin phosphate, by Merck Group (1 mentions)
4 protocols using gallic acid
1
Comprehensive Reagent Sourcing for Multidisciplinary Research
2
Cytotoxicity Evaluation of Bioactive Compounds
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Clindamycin phosphate, Penicillin/Streptomycin (P/S), 0.25% trypsin/EDTA, Veratrine alkaloid, RPMI-1640, Fetal bovine serum (FBS), Bismuth III nitrate, atropine, quercetin, andrographolide, Folin-Ciocalteus, and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reagents were purchased from Sigma Aldrich, USA. Other chemicals such as dimethyl sulphoxide (DMSO), bromocresol green, and gallic acid were purchased from Biobasic, Canada, while absolute ethanol was from VWR Chemicals, France.
3
Quantifying Total Phenolic Content in Brahmi
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Total phenolic content (TPC) in the Brahmi products was evaluated using the Folin-Ciocalteu assay [26 (link)] as a mixture of gallic acid (Bio Basic, Canada) or Brahmi products' extracts (15 μL), deionized water (240 μL), and Folin-Ciocalteu reagent (15 μL). The mixtures were gently mixed and incubated for 5 min. Subsequently, 10 % w/v Na2CO3 (70 μL) was added, and the mixtures were continuously mixed and incubated at room temperature in the dark for 60 min. After incubation, the absorbances of the mixtures were measured at 750 nm using a microplate reader (BioTek Synergy H1, Agilent Technologies, USA). The TPC in the Brahmi products' extracts was then calculated using a linearity equation obtained from the standard calibration curve of absorbances against concentrations of gallic acid (ranging from 0 to 400 μg/mL, Fig. S2 ), and expressed in milligrams of gallic acid equivalent (GAE) per gram of dry Brahmi products’ powder (mg GAE/g).
4
Comprehensive Reagent Procurement for Research
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Copper sulfate pentahydrate, ferric chloride, potassium ferricyanide, sodium nitrate and sodium nitrite were purchased from Ajax Finechem (New South Wales, Australia). CIP hydrochloride and gallic acid were from Bio Basic Inc. (Markham, Canada). Epigallocatechin gallate, quercetin, and 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) were from Sigma-Aldrich (Steinheim, Germany). Hydrochloric acid (HCl) was from QRëC (Auckland, New Zealand. Folin-Ciocalteu reagent was from Loba Chemie (Mumbai, India). Aluminum chloride hexahydrate, bromophenol blue, and calcium carbonate were from Kemaus (New South Wales, Australia). Trichloroacetic acid and vanillin were from Merck (Damstadt, Germany). Sodium hydroxide (NaOH) was from RCI Lab-scan (Bangkok, Thailand). All other solvents and chemicals were of analytical grade.
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