Ab66340

Manufactured by Abcam

Sourced in United States

Ab66340 is a monoclonal antibody that recognizes the full-length human alpha-synuclein protein. This antibody can be used for the detection of alpha-synuclein in various applications such as Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

Ab76959, by Abcam (1 mentions) Ab88078, by Abcam (1 mentions) Protein assay, by Bio-Rad (1 mentions) Monoclonal anti α tubulin antibody, by Merck Group (1 mentions) Immobilon p transfer membrane, by Merck Group (1 mentions) Ab155102, by Abcam (1 mentions) Ab45766, by Abcam (1 mentions) Ab38007, by Abcam (1 mentions) Ab195046, by Abcam (1 mentions)

3 protocols using ab66340

Quantification of Glycolytic Enzymes

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Tissues were homogenised in lysis buffer (20 mM Tris, 1 mM EDTA and 0.25 mM sucrose, pH 7.4) and ultracentrifuged to obtain the membrane fractions. The amount of protein in the homogenate was quantified using the Bio-Rad Protein Assay, based on the Bradford method. Equivalent amounts of protein were resolved on an 8–12% SDS-PAGE and transferred onto a PVDF membrane (Immobilon-P transfer membrane, Millipore). Glycogen synthase was detected using the Abcam (EP817Y, ab40810) rabbit monoclonal anti-gys1 primary antibody. GLUT-4 was detected using the Abcam (mAbcam65267) anti-GLUT-4 primary antibody. Hexokinase II was detected using the Abcam (ab76959) mouse monoclonal Anti-Hexokinase II antibody. Glucose-6-phosphate isomerase was detected using the Abcam [1B7D7] (ab66340) mouse monoclonal Anti-Glucose-6-phosphate isomerase antibody. Glycogen phosphorylase was detected using the Abcam (ab88078). Monoclonal Anti-α-Tubulin antibody (Sigma–Aldrich) probed with the Polyclonal Rabbit Anti-mouse Immunoglobulins/HRP secondary antibody (Dako Cytomation) were used for the determination of the loading controls.

Xirouchaki C.E., Mangiafico S.P., Bate K., Ruan Z., Huang A.M., Tedjosiswoyo B.W., Lamont B., Pong W., Favaloro J., Blair A.R., Zajac J.D., Proietto J, & Andrikopoulos S. (2016). Impaired glucose metabolism and exercise capacity with muscle-specific glycogen synthase 1 (gys1) deletion in adult mice. Molecular Metabolism, 5(3), 221-232.

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Comprehensive Protein Analysis Protocol

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Western blot analysis was performed as described before with antibodies specific for β‐actin (ab125402), β‐catenin (ab32572), RBM15 (ab244374), ENO1 (ab155102), GPI (ab66340), PGK1 (ab38007), Flag (ab45766, Abcam, USA), ALDOA (sc‐390733) and IGF2BP1 (sc‐166344, Santa Cruz Biotechnology, USA).

Yang F., Liu Y., Xiao J., Li B., Chen Y., Hu A., Zeng J., Liu Z, & Liu H. (2022). Circ‐CTNNB1 drives aerobic glycolysis and osteosarcoma progression via m6A modification through interacting with RBM15. Cell Proliferation, 56(1), e13344.

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Protein Expression Profiling of Cellular Metabolism

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Briefly, total protein was extracted, qualified by BCA protein assay reagent, separated by SDS-PAGE, and detected by immunoblotting with specific antibodies. Antibodies against FDFT1 (Abcam, ab195046), mTOR (Abcam, ab10268), AKT (Cell Signaling, #9272), vinculin (Cell Signaling, #4650), HIF1α (Novus, NB100-105), GLUT1 (HIF-Proteintech, 66290-1-lg), LDHA (Proteintech, 19987-1-AP), HK2 (Proteintech, 22029-1-AP), PGK1 (Proteintech, 17811-1-AP), and GPI (Abcam, ab66340) were purchased from the designated manufacturers. Vinculin was used as the loading control.

Weng M.L., Chen W.K., Chen X.Y., Lu H., Sun Z.R., Yu Q., Sun P.F., Xu Y.J., Zhu M.M., Jiang N., Zhang J., Zhang J.P., Song Y.L., Ma D., Zhang X.P, & Miao C.H. (2020). Fasting inhibits aerobic glycolysis and proliferation in colorectal cancer via the Fdft1-mediated AKT/mTOR/HIF1α pathway suppression. Nature Communications, 11, 1869.

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