Transwell in vitro invasion 24-well chambers (Corning) were used per the manufacturer's instructions. Briefly, the filter was pre-coated with 50 μL Matrigel (BD Biosciences), which was allowed to solidify at 37°C for 1 h. Cells (4.0×104 cells/well for HUVECs, 2.0×104 cells/well for MDA-MB-231) were then placed in the top chambers and treated with different concentrations of YLL545 or sorafenib. Cells were then allowed to migrate for 16 h. Non-migrated cells were scraped with a cotton swab, and migrated cells were fixed with 20% methanol and stained with 0.5% crystal violet. The cells were counted and photographed under a light microscope (Olympus). Cell migration is expressed as a percentage of the migration observed in vehicle-treated cells.
Transwell in vitro invasion 24 well chambers
Manufactured by Corning
Sourced in United States
Transwell in vitro invasion 24-well chambers are a laboratory equipment product designed for cell invasion assays. The product consists of a 24-well plate with inserts that create a barrier between the upper and lower chambers, allowing for the study of cell migration and invasion through this barrier.
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Lab products found in correlation
3 protocols using transwell in vitro invasion 24 well chambers
1
Transwell Invasion Assay for Cell Migration
2
Transwell Invasion Assay for Bone Cells
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Transwell in vitro invasion 24-well chambers (Corning, USA) were used following the manufacturer's instruction. The membrane of each well was precoated with 500μL BD Matrigel (BD Biosciences, Bedford, MA). 2.5×10 4 143B or MG63 cells in 200μL serum-free medium were placed into the upper chamber, BMP-2 or Spp24 was added into the upper chamber as previously described. 500μL of medium containing 10% FBS was placed into the lower compartment. The transwell chambers were incubated for 24 h at 37°C in 95% air with 5% CO 2 . The membranes were xed with methanol and cell penetration through the membrane was detected using crystal violet staining. Cell penetration was quanti ed by counting the numbers of cells in ve microscopic elds (at x200 magni cation) per lter. The studies were conducted in triplicate.
3
Transwell Invasion Assay for Osteosarcoma
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Transwell in vitro invasion 24-well chambers (Corning, USA) were used following the manufacturer's instruction. The membrane of each well was precoated with 500μL BD Matrigel (BD Biosciences, Bedford, MA). 2.5×10 4 143B or MG63 cells in 200μL serum-free medium were placed into the upper chamber, BMP-2 or Spp24 was added into the upper chamber as previously described. 500μL of medium containing 10% FBS was placed into the lower compartment. The transwell chambers were incubated for 24 h at 37°C in 95% air with 5% CO 2 . The membranes were fixed with methanol and cell penetration through the membrane was detected using crystal violet staining. Cell penetration was quantified by counting the numbers of cells in five microscopic fields (at x200 magnification) per filter. The studies were conducted in triplicate.
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