Diazoxide (Tocris) was initially dissolved in dimethyl sulfoxide (DMSO; Sigma) to a final concentration of 20 mg mL−1. Stock solution (with and without drug) were diluted in Buffer Saline (D-PBS; Invitrogen) prior to injection for drug and vehicle-control injections. C57B/J6 pups were given a single injection daily in the nape of the neck with either vehicle or Diazoxide (10, 20, 50 or 100 mg kg−1) from P2 to P12. This age period is equivalent to the vulnerable period of PWMI (Back and Rivkees, 2004 ; Craig et al., 2003 (link)). In preliminary studies, the dose of 100 mg kg−1 of Diazoxide caused death in 84% of mice and therefore was not used. Individual daily doses per pup were based on the average litter weight per cage.
Diazoxide
Manufactured by Bio-Techne
Sourced in United Kingdom
Diazoxide is a laboratory reagent used for research purposes. It is a potassium channel opener that can modulate cellular processes. The core function of Diazoxide is to act as a tool for scientific investigation, without making claims about its intended use or application.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Perfluorooctanesulfonic acid, by Merck Group (1 mentions)
Bisphenol f, by Merck Group (1 mentions)
Bisphenol a, by Merck Group (1 mentions)
Bisphenol s, by Merck Group (1 mentions)
Di 2 ethylhexyl phthalate, by Merck Group (1 mentions)
3 isobutyl 1 methyl xanthine (ibmx), by Merck Group (1 mentions)
Nifedipine, by MP Biomedicals (1 mentions)
Repaglinide, by Bio-Techne (1 mentions)
Glimepiride, by MedChemExpress (1 mentions)
Nateglinide, by Bio-Techne (1 mentions)
Pnu 37883a, by Bio-Techne (1 mentions)
P1075, by Bio-Techne (1 mentions)
Levcromakalim, by Bio-Techne (1 mentions)
4 protocols using diazoxide
1
Diazoxide Treatment in Perinatal Brain Injury
2
Preparation of Chemical Stock Solutions
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Bisphenol A (BPA, Cat No 239658), bisphenol S (BPS, Cat No 103039), bisphenol F (BPF, Cat No B47006), Di(2-ethylhexyl) phthalate (DEHP, Cat No 36735), perfluorooctanesulfonic acid (PFOS, Cat No 77283), cadmium chloride (CdCl2, Cat No 202908), and 1,1-Dichloro-2,2-bis(4-chlorophenyl)ethene, 4,4′-DDE (4,4′-DDE, Cat No 35487), and IBMX (Cat No I-5879), were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Diazoxide (Cat no 0964) was purchased from Tocris Cookson (Bristol, UK). All chemicals were dissolved in dimethyl sulfoxide (DMSO) to prepare the stock solution, except for CdCl2, which was dissolved in water. The amount of DMS was maintained at less than 0.03%.
3
Pancreatic Manganese Uptake Modulation
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Pancreatic 52Mn2+ uptake was stimulated using glucose and glibenclamide (Tocris Biosciences), which is known to promote insulin release in β-cells via blockade of ATP-sensitive K+ channels (KATP). Mice were injected intraperitoneally (i.p.) with 100 µL of 1 g/kg glucose, or glucose plus 5 mg/kg glibenclamide in PBS, 15 min before the i.v. injection of 0.74–1.85 MBq (20 μCi) of 52Mn2+. VDCC blockade was achieved via i.p. injection of 20 mg/kg nifedipine (MP Biomedicals) dissolved in DMSO. To activate KATP channels, mice received an i.p. injection of 20 mg/kg diazoxide (Tocris Biosciences) in PBS, a clinically used KATP agonist, 15 min before injection of 0.74 MBq (20 μCi) of 52Mn2+. Whole-body PET scans were acquired 1 h after the injection of the radiotracer, after which ex vivo biodistribution analysis was performed.
4
Potassium Channel Modulators in Cell Assays
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BTC/AM was obtained from BioNordika (Herlev, Denmark). Diazoxide, levcromakalim, nateglinide, P1075, PNU-37883A, repaglinide, Y-26763, and ZM226600 were purchased from Tocris (Bristol, UK). BMS-191095, gliclazide, and glimepiride were purchased from MedChemExpress (Sollentuna, Sweden). All other chemicals were obtained from Sigma-Aldrich. Test compounds were dissolved in anhydrous DMSO to generate 10 mM stock solutions and diluted in assay buffer before use. The final concentration of DMSO used was less than or equal to 1 % (v/v).
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