The dried meshes were sputter coated with 4-nm gold and imaged on a Quanta 200 FEG MKII scanning electron microscope (FEI Inc., Hillsboro, OR) under high vacuum at 5 kV. The average fiber diameter was quantified from 50 randomly selected fibers in the micrograph acquired under 1000× magnification using ImageJ (NIH).
Quanta 200 feg mkii
Manufactured by Thermo Fisher Scientific
The Quanta 200 FEG MKII is a field emission scanning electron microscope (FESEM) designed for high-resolution imaging and analysis of a wide range of sample types. It features a field emission gun source, which provides high brightness and small probe size for enhanced resolution and imaging performance.
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Lab products found in correlation
5 protocols using quanta 200 feg mkii
1
Scanning Electron Microscopy Analysis of Fiber Diameter
2
Comparing Cotyledon Vein Patterns in Transgenic and WT A. thaliana
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To compare cotyledon vein patterns of transgenic and WT A. thaliana plants, the cotyledons were bleached with 75% (v/v) ethanol and observed using stereomicroscopy. For cotyledon epidermal cell analysis, the cotyledons of transgenic and WT A. thaliana plants were fixed in FAA fixative solution (38% formalin, 50% ethanol, and glacial acetic acid). After critical point drying (CPD), the samples were coated with an Edwards E-1010 ion sputter golden coater and examined with a scanning electron microscope (FEI Quanta 200 FEG MKII).
3
Tomato Leaf Ultrastructure Visualization
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Infected and control tomato leaves were cut into ∼1-cm2 size pieces and fixed with 3% glutaraldehyde in 0.2-M sodium phosphate buffer (pH 7.4) overnight at 4°C. After rinsing the samples in same buffer, they were dehydrated in increasing concentration of ethanol (50, 70, 85 and 95%) for 30 min in each solution and were kept in absolute ethanol until attaching on SEM stubs. The prepared specimens were directly examined in a SEM (FEI Quanta 200 FEG MKII) at 5–10 kV.
4
Transmission and Scanning EM of NPs
5
Scanning Electron Microscopy of Imaginal Discs
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Imaginal discs from 3rd instar larvae were dissected and fixed in 2.5% (v/v) glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.2) for 1 h at RT, and then at 4 °C overnight and post-fixed in 1% (w/v) osmium tetroxide for 1 h at RT. The imaginal discs were dehydrated through a graded series of ethanol to 100% and then transferred into porous sample holders and dried at a critical point in liquid CO2. The dried samples were transferred to aluminum SEM stubs coated with adhesive carbon tape, the edges were painted with silver conductive paste and the SEM stubs were sputter-coated with Au/Pd (80/20). The specimens were then examined using an FEI Quanta 200 FEG MKII scanning electron microscope at 10 kV accelerating voltage.
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