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2 protocols using flufenamic acid

1

Diffusion Study of Non-Steroidal Anti-Inflammatory Drugs

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The Franz diffusion cells (surface area 4.9 cm2, receptor phase volume 20 mL) were obtained from Crown Glass (Somerville, NJ, USA) and the water bath was from Fisher Scientific (Pittsburg, PA, USA). Diclofenac, Ibuprofen, Ketoprofen, and Naproxen were purchased from TCI (Tokyo, Japan). Nabumetone, Piroxicam, Diflunisal, and Theophylline were purchased from Sigma-Aldrich (St. Louis, MO, USA). Flufenamic Acid was purchased from Acros Organics (Geel, Belgium). Aspirin was purchased from Eastman Kodak Chemicals (Rochester, NY, USA). Silicone membranes (0.254 mm) were purchased from Pillar Surgical (La Jolla, CA, USA) and all solvents were purchased from Fisher Scientific.
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2

Evaluating Modulation of Ion Channels

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Chemicals are commercially available: DCPIB (Sigma-Aldrich, #SML2692-25MG), ARL 67156 trisodium salt (Tocris, #1283), S1P (Cayman, #62570), W146 (Tocris, #36024), JTE013 (Tocris, #2392), BAPTA AM (Cayman, #15551), diphenyleneiodonium (Tocris, #0504), ATP (Sigma-Aldrich, #A2382-5G), lipopolysaccharide (LPS) (Sigma-Aldrich, #L2880-100MG), nigericin (Cayman, 11437), dicumarol (Acros Organics, #204120050), chenodiol (VWR 21947-5G), tioxolone (Sigma-Aldrich, #217077), tolfenamic acid (Sigma-Aldrich, #T0535-5G), flufenamic acid (Acros Organics, #165920100), zafirlukast (TCI America, #Z0029-100MG), 4-hydroxycoumarin (Acros Organics, #121100050), and warfarin (Agilent, #PST-1000). All chemicals were dissolved with the manufacturers’ instructions. Dicumarol stock solution (10 to 20 mM) was made in dimethyl sulfoxide with brief sonication (2 to 5 min) and heating (50°C).
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