Wt1 creert2 mice

Manufactured by Jackson ImmunoResearch

The WT1-CreERT2 mice are a genetically modified mouse model that expresses a tamoxifen-inducible Cre recombinase under the control of the Wilms' Tumor 1 (WT1) gene promoter. The Cre recombinase is fused to a mutated estrogen receptor ligand-binding domain, which allows for temporal control of Cre activity upon administration of tamoxifen.

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Lab products found in correlation

Icr strain mice, by CLEA Japan (1 mentions) Sesame oil, by Nacalai Tesque (1 mentions) R26mtmg mice, by Jackson ImmunoResearch (1 mentions) Ubc cre ert2 mice, by Jackson ImmunoResearch (1 mentions) R26 lsl tdtomato mice, by Jackson ImmunoResearch (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Ot 2 mice, by Jackson ImmunoResearch (1 mentions)

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Wt1-CreERT2 (7 citations)

3 protocols using wt1 creert2 mice

Generation of Transgenic Mouse Lines

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Generation of floxed Smad4 alleles and Stra8 knockout mice has been described previously [48 (link),55 (link)]. Stella-MerCreMer was established previously [33 (link)]. Rosa-CreERT2 mice were purchased from Artemis Pharmaceuticals GmbH. WT1-CreERT2 mice were purchased from Jackson Laboratory. Gt(ROSA)26Sor^tm1Sor/J and CAG-floxed-CAT-EGFP mouse lines were established previously [56 (link),57 (link)]. All of these mice lines were kept in a mixed background. TM was diluted in sesame oil (Nacalai Tesque) at a concentration of 10 mg/ml, and 0.5 ml of the diluted TM was injected each time. To induce recombination in Stella-MerCreMer mice, TM was injected at E9.5 and E10.5. To induce recombination in Rosa-CreERT2 or WT1-CreERT2 mice, TM was injected at E10.5 and E11.5. ICR strain mice (Clea Japan) were used as control in some cases (S1A, S5D and S5E and S6B Figs).

Wu Q., Fukuda K., Kato Y., Zhou Z., Deng C.X, & Saga Y. (2016). Sexual Fate Change of XX Germ Cells Caused by the Deletion of SMAD4 and STRA8 Independent of Somatic Sex Reprogramming. PLoS Biology, 14(9), e1002553.

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Genotyping Transgenic Mouse Lines

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Tetracycline-regulated i-TERT^ci transgenic mice, WT1^CreERt2 mice (The Jackson Laboratory, stock# 010912), R26^mTmG mice (The Jackson Laboratory, stock# 007576), UBC^CreERt2 mice (The Jackson Laboratory, stock# 008085), R26^confetti mice (The Jackson Laboratory, stock# 013731), and TERT^KO mice (The Jackson Laboratory, stock# 005423) were previously described^{13 (link),18 (link),27 (link),28 (link),43 (link)–45 (link)}. Mice were PCR-genotyped using the following oligonucleotide pairs: 5’-CGCCCAGAAGCTTGGTGTAG−3’, 5’-GCTCCATGGCGATGACTTAG-3’ (actin-rtTA + ); 5’-GGATGTACTTTGTTAAGGCAGCA-3’, 5’-ACAACGGAGTTCCTCAGTGC-3’ (tetop-TERT^ci + ); 5’-ATCGCAGGAGCGGAGAAC-3’, 5’-GCAAACGGACAGAAGCATTT-3’ (WT1^CreERt2); 5’-CTCTGCTGCCTCCTGGCTTCT-3’, 5’-CGAGGCGGATCACAAGCAATA-3’, 5’-TCAATGGGCGGGGGTCGTT-3’ (R26^mTmG); 5’-GACGTCACCCGTTCTGTTG-3’, 5’-AGGCAAATTTTGGTGTACGG-3’ (UBC^CreERt2); 5’-GAATTAATTCCGGTATAACTTCG-3’, 5’-AAAGTCGCTCTGAGTTGTTAT-3’, 5’-CCAGATGACTACCTATCCTC-3’ (R26^Confetti); 5’-CCCCAGGCGCCGCACAAAGG-3’, 5’- GGTCCTGGCTGTTTTCTAAG-3’, 5’-CTGGATTCATCGACTGTGGC-3’ (TERT^KO).

Montandon M., Hamidouche T., Yart L., Duret L.C., Pons C., Soubeiran N., Pousse M., Cervera L., Vial V., Fassy J., Croce O., Gilson E, & Shkreli M. (2022). Telomerase is required for glomerular renewal in kidneys of adult mice. NPJ Regenerative Medicine, 7, 15.

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Murine Pancreatic Tumor Initiation and Progression

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KIC and KPfC mice were generated as previously described (Hingorani et al., 2003 (link); Hingorani et al., 2005 (link)). Mice were sacrificed when they had early-stage tumors (40-day-old) or late-stage (60-day-old) tumors for KIC and KPfC mice. The KIC mice are on a mixed background (C57BL/6 with FVB). The KPfC mice are a pure C57BL/6 genetic background. For staining and lineage tracing assays, 3–6 mice (male and female) were used per group. Normal pancreata were obtained from Cre-negative littermates of the KIC or KPfC mice. 6-week-old mice male and female mice were purchased from The Jackson Laboratory, including C57BL/6J mice (JAX stock #000664), Wt1^CreERT2 mice (JAX stock #010912), R26^LSL-tdTomato mice (JAX stock #007914), immortomice (JAX stock #032619) and OT II mice (JAX stock #004194). Wt1^CreERT2 mice were backcrossed into a C57BL/6 background. All animals were housed in a pathogen-free facility with 24-hr access to food and water. Animal experiments in this study were approved by and performed in accordance with the institutional animal care and use committee at the UTSW Medical Center at Dallas. Mice were euthanized by cervical dislocation under anesthesia.

Huang H., Wang Z., Zhang Y., Pradhan R.N., Ganguly D., Chandra R., Murimwa G., Wright S., Gu X., Maddipati R., Müller S., Turley S.J, & Brekken R.A. (2022). Mesothelial cell-derived antigen-presenting cancer-associated fibroblasts induce expansion of regulatory T cells in pancreatic cancer. Cancer cell, 40(6), 656-673.e7.

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