C13440 orca flash4.0 v3

Unless otherwise stated, live worms were placed onto 2% agar pads and anaesthetised using 0.2% levamisole. Images were captured using either a Zeiss Axioskop 2 plus microscope with a Hamamatsu ORCA-ER digital camera C4742-95 and Volocity 6.3 software (Macintosh version) for image acquisition; or an ApoTome.2 Zeiss microscope with a Hamamatsu digital camera C13440 ORCA-Flash4.0 V3 and Zen software. A constant exposure time was maintained between samples in fluorescence intensity comparisons. Brightness and contrast were adjusted equally across the entire image, and where applicable applied equally to controls. Where Nomarski and fluorescence images were superimposed, brightness and contrast were adjusted separately prior to superimposition.

A sample of 1% SDS-treated nematodes was placed on 2% agar pads and anaesthetized in a drop of 0.2% levamisole, and a cover slip placed on top. Images were captured using a Zeiss ApoTome.2 microscope with a Hamamatsu digital camera C13440 ORCA-Flash4.0 V3 and Zen software. Recovering dauers were identified by virtue of one or more of the following characteristics: increased diameter of the pharynx, twitching in the terminal bulb of the pharynx (recovery of pharyngeal pumping function) and increased body length.

Live nematodes were placed on 2% agar pads, and anaesthetised with a drop of 0.2% levamisole. Images were captured using either a Zeiss Axioskop 2 plus microscope with a Hamamatsu ORCA-ER digital camera C4742-95 and Velocity 6.3 software (Macintosh version) for image acquisition; or an ApoTome.2 Zeiss microscope with a Hamamatsu digital camera C13440 ORCA-Flash4.0 V3 and Zen software. Brightness and contrast were adjusted equally across the entire image, and where applicable applied equally to controls.