ACE (from rabbit lung), N-Hippuryl-His-Leu tetrahydrate (HHL), Griess reagent, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), gastrointestinal enzymes (pepsin, α-chymotrypsin and trypsin) and Ang II were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Specific antibodies against inducible nitric oxide synthase (iNOS; sc-7271), GAPDH (sc-25778), p-p38 (sc-7973), p38 (sc-7149), p-c-Jun N-terminal kinase (JNK; sc-6254), JNK (sc-7345), p-extracellular signal-regulated kinase (ERK; sc-7383), ERK (sc-292838) and ET-1 (sc-21625) were all purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), and 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) and Hoechst 33342 were obtained from Invitrogen Life Technologies (Carlsbad, CA, USA). The HiPrep™ 16/10 DEAE FF anion exchange column was purchased from GE Healthcare (Buckinghamshire, UK). The other chemicals and reagents were of analytical grade.
Hiprep 16 10 deae ff anion exchange column
Manufactured by GE Healthcare
Sourced in United Kingdom, Sweden
The HiPrep™ 16/10 DEAE FF anion exchange column is a laboratory equipment product designed for the separation and purification of biomolecules. It features a diethylaminoethyl (DEAE) anion exchange medium packed in a 16 x 100 mm column format. This column is intended for use in chromatographic techniques to facilitate the capture and fractionation of negatively charged molecules, such as proteins, enzymes, and nucleic acids.
Automatically generated - may contain errors
Lab products found in correlation
Sc 7271, by Santa Cruz Biotechnology (1 mentions)
P c jun n terminal kinase, by Santa Cruz Biotechnology (1 mentions)
α chymotrypsin, by Merck Group (1 mentions)
2 7 dichlorodihydrofluorescein diacetate dcfh da, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Gapdh sc 25778, by Santa Cruz Biotechnology (1 mentions)
Trypsin, by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
Akta purifier system, by GE Healthcare (1 mentions)
2 protocols using hiprep 16 10 deae ff anion exchange column
1
Angiotensin-Converting Enzyme Isolation
2
Purification of ACE Inhibitory Peptides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The ultrafiltration fraction with the highest ACE inhibitory activity was subjected to further purification through a HiPrep 16/10 DEAE FF anion exchange column (GE Healthcare) using an AKTA purifier system (GE Healthcare, Uppsala, Sweden) at a concentration of 20 mg/ml. The column was equilibrated with 5 column volumes of 20 mM sodium acetate buffer (pH 4). The sample was then injected and eluted at a linear gradient of 2 M NaCl (0-30%) in the same buffer at a flow rate of 60 ml/h. The absorbance eluent was monitored at 280 nm. The fraction was collected to a volume of 10 ml, desalted and lyophilized before its ACE inhibitory activity was determined.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!