Clampfit 10

Analysis was performed using Clampfit 10.7, OriginPro 2016 and GraphPad Prism 4. Fit-derived parameters were compared with Student’s t-tests or one-way ANOVA. Current-voltage curves were compared with a two-way ANOVA, where the dependent variable was ‘current’ and the independent variables ‘voltage’ and ‘gene variant’. Data are presented as mean ± standard error of the mean (SEM) with significance level at P < 0.05.

Effects of mutation on splicing were analysed using Alamut Visual v2.7.1 (Interactive Biosoftware, Rouen, France). Current recordings were analysed using Clampfit 10.7, OriginPro 2016 and GraphPad softwares. All data is presented as mean ± SEM. Patch clamp data was analysed using one-way ANOVA, except for open-state inactivation data that was studied using two-way ANOVA, and the peak current density and recovery time-constant data that were analysed using Kruskal-Wallis ANOVA. The gating pore current amplitude data were compared at −90 mV using Kruskal-Wallis ANOVA. Means were compared using Bonferroni post tests for one and two way ANOVA analyses or using Dunn’s multiple comparison tests for Kruskal-Wallis analyses. Significance was established at p < 0.05. Some of the raw data for R222W and wild-type main pore currents and for R222W, R222G and wild-type gating pore currents has been published^{14 (link)}.