Polyethylene glycol 400 (PEG 400) and heparin sodium were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pentobarbital sodium was obtained from SCI Phar-matech (Toayuan, Taiwan). S. chinensis was purchased from Kaiser Pharmaceutical Co., Ltd. (Tainan, Taiwan). Methanol, ethyl acetate and formic acid were obtained from E. Merck (Darmstadt, Germany). Triply deionized water (Millipore, Bedford, MA, USA) was used for all preparations.
Triply deionized water
Manufactured by Merck Group
Sourced in United States
Triply deionized water is a highly purified form of water that has undergone a three-stage deionization process. It is a laboratory-grade water that has been stripped of nearly all dissolved ions, making it suitable for use in various scientific and analytical applications where high-purity water is required.
Automatically generated - may contain errors
Lab products found in correlation
Polyethylene glycol 400 peg 400, by Merck Group (4 mentions)
Heparin sodium, by Merck Group (3 mentions)
Methanol, by Merck Group (3 mentions)
Formic acid, by Merck Group (2 mentions)
Biochanin a, by LGC (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Diethylstilbestrol, by Merck Group (2 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Urethane, by Merck Group (1 mentions)
Dextrose, by Merck Group (1 mentions)
Gs 441524, by Selleck Chemicals (1 mentions)
Cabozantinib, by Merck Group (1 mentions)
Cyclosporin a, by Merck Group (1 mentions)
Lenvatinib, by Merck Group (1 mentions)
Potassium dihydrogen phosphate monohydrate, by Merck Group (1 mentions)
7 protocols using triply deionized water
1
Extraction and Analysis of S. chinensis
2
Quantification of Remdesivir and its Metabolite
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Remdesivir was provided by Formosa Laboratories Inc. (Taoyuan, Taiwan). GS-441524 was purchased from Selleck Chemicals (Houston, TX, USA). Formic acid, polyethylene glycol 400 (PEG400), and urethane were obtained from Sigma-Aldrich (St. Louis, MO, USA). Critic acid, dextrose, sodium chloride, and sodium citrate were purchased from Merck (Darmstadt, Germany). MS-grade acetonitrile (ACN) was acquired from J.T. Baker Inc. (Phillipsburg, NJ, USA). Triply deionized water (Millipore, Bedford, MA, USA) was used to prepare the stock solutions and mobile phase. Standard stock solutions of remdesivir and GS-441524 were prepared using 10% DMSO in ACN, at a concentration of 1 mg/mL, and stored at -20°C. Calibration curves of remdesivir and GS-441524 were prepared using blank matrices (blood, fetus, placenta, and amniotic fluid) as 5 μL standard stock solutions added into (spike) 45 μL blank matrices, to generate solutions with concentrations of 0.5, 1, 5, 10, 50, and 100 ng/mL and 5, 10, 50, 100, 250, and 500 ng/mL, respectively.
3
Cabozantinib Quantification in Biological Samples
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Cabozantinib was purchased from Sigma‒Aldrich (St. Louis, MO, USA). Biochanin A, as an internal standard, was purchased from Toronto Research Chemicals Inc. (North York, ON, Canada). Polyethylene glycol 400 (PEG 400) and heparin sodium were purchased from Sigma-Aldrich. Pentobarbital sodium was obtained from SCI Pharmtech (Taoyuan, Taiwan). The solvents and reagents for chromatography were purchased from J.T. Baker (Phillipsburg, NJ, USA) and Merck (Darmstadt, Germany). Standard solutions of lenvatinib and Biochanin A were stored in methanol at −20 °C. Triply deionized water from Millipore (Bedford, MA, USA) was used for all preparations.
4
In Vitro Cytochrome P450 Enzyme Assay
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The following compounds were obtained from Alfa Aesar (Heysham, UK): 3′,4′,7,8-TMF (78-TMF),3′,4′,5,7-TMF (57-TMF), 3-hydroxy-2′,4′,5′,7-TMF (3H7-TMF), and 3-hydroxy-2′,4′,5′,6-TMF (3H6-TMF). All other chemicals used were of analytical grade. Liquid chromatographic grade solvents and reagents were provided by Mallinckrodt Baker (Phillipsburg, NJ, USA). All preparations were made with triply deionized water (Millipore, Bedford, MA, USA). P450 Baculosomes® (microsomes prepared from insect cells containing a single human P450 and P450 reductase), control Baculosomes® (microsomes prepared from insect cells infected with a control virus), regeneration system (glucose-6-phosphate dehydrogenase and glucose–6–phosphate), NADP+, Vivid® substrates, and assay buffers were obtained from Invitrogen Corp. (Madison, WI, USA). Corning provided 384 low–volume black polystyrene plates for the assays (Acton, MA, USA).
5
Simultaneous Determination of Sorafenib and Gentiana Extract
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Sorafenib (BAY 43-9006; purity higher than 99% by HPLC) was kindly provided by Bayer® Pharmaceutical Co., Ltd. (Kaiser-Wilhelm-Allee, Leverkusen, Germany). The Radix Gentianae formulation was purchased from Sun Ten Pharmaceutical Co., Ltd. (Xindian, Taiwan). The internal standard (diethylstilbestrol; purity = 99% for HPLC) was purchased from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Liquid chromatography-grade acetonitrile, methanol and potassium dihydrogen phosphate monohydrate (KH2PO4·H2O) were purchased from E. Merck (Darmstadt, Germany). Triply deionized water (Millipore, Bedford, MA, USA) was used for all aqueous solutions. All of the other reagents were of analytical grade.
6
Quantitative Analysis of Lenvatinib and Biochanin A
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Lenvatinib and cyclosporin A were purchased from Sigma-Aldrich (St. Louis, MO, USA). As an internal standard, biochanin A was purchased from Toronto Research Chemicals Inc. (North York, ON, Canada). Polyethylene glycol 400 (PEG 400) and heparin sodium were purchased from Sigma-Aldrich. Pentobarbital sodium was obtained from SCI Pharmtech (Taoyuan, Taiwan). The solvents and reagents for chromatography were purchased from J.T. Baker (Phillipsburg, NJ, USA) and Merck (Darmstadt, Germany). Standard solutions of Lenvatinib and biochanin A in methanol were stored at −20 °C. Triply deionized water from Millipore (Bedford, MA, USA) was used for all preparations.
7
Sorafenib Quantification in H. diffusa
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Sorafenib
(BAY 43-9006) of over 99% purity level was obtained by Bayer Pharmaceutical.
(Kaiser-Wilhelm-Allee, Leverkusen, Germany). H. diffusa (production batch number, BP6005270) was purchased from Sheng-Chang
Pharmaceutical. (Taoyuan, Taiwan). The internal standard (diethylstilbestrol)
with a purity higher than 99% was obtained from Sigma-Aldrich Chemicals
(St. Louis, MO, USA). Other liquid chromatography grade reagents,
including methanol, acetonitrile, and potassium dihydrogen phosphate
monohydrate, were all obtained from E. Merck (Darmstadt, Germany).
All reagents used in the HPLC experiment were prepared with triply
deionized water (Millipore, Bedford, MA, USA).
Sorafenib and
diethylstilbestrol were dissolved in methanol and diluted with 50%
methanol to the specified concentrations as stock solutions. The calibration
and internal standard solution were stored at −20 °C for
subsequently analysis. Before drug administration for the animal experiment,
the H. diffusa extract was prepared
with triply deionized water.
(BAY 43-9006) of over 99% purity level was obtained by Bayer Pharmaceutical.
(Kaiser-Wilhelm-Allee, Leverkusen, Germany). H. diffusa (production batch number, BP6005270) was purchased from Sheng-Chang
Pharmaceutical. (Taoyuan, Taiwan). The internal standard (diethylstilbestrol)
with a purity higher than 99% was obtained from Sigma-Aldrich Chemicals
(St. Louis, MO, USA). Other liquid chromatography grade reagents,
including methanol, acetonitrile, and potassium dihydrogen phosphate
monohydrate, were all obtained from E. Merck (Darmstadt, Germany).
All reagents used in the HPLC experiment were prepared with triply
deionized water (Millipore, Bedford, MA, USA).
Sorafenib and
diethylstilbestrol were dissolved in methanol and diluted with 50%
methanol to the specified concentrations as stock solutions. The calibration
and internal standard solution were stored at −20 °C for
subsequently analysis. Before drug administration for the animal experiment,
the H. diffusa extract was prepared
with triply deionized water.
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