Tail vein fibroblasts from each group were isolated by cutting 5 cm of tail from 2-month-old mice, peeling the dermis and mincing the tail tips into 1 cm pieces. A pair of pieces was plated on a 600 mm collagen I coated dish (BD Biosciences, Bedford, MA, USA) with 5 mL DMEM containing 10% FBS (Sigma-Aldrich, St. Louis, MO, USA). After 5 days of incubation, fibroblasts migrated out of the tail pieces and were transferred to new dishes and allowed to proliferate.
600 mm collagen 1 coated dish
Manufactured by BD
Sourced in United States
The 600 mm collagen I coated dish is a cell culture dish designed to provide a suitable extracellular matrix for cell attachment and growth. The dish is coated with collagen I, a major structural protein found in the human body. This coating promotes cell adhesion and supports the in vitro culture of various cell types.
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2 protocols using 600 mm collagen 1 coated dish
1
Tail Vein Fibroblast Isolation Protocol
2
Isolation of Tail Vein Fibroblasts
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Tail vein fibroblasts from all groups were isolated by cutting 5 cm of tail from 2-month-old mice, peeling the dermis and mincing the tail tips into 1-cm pieces. A pair of pieces was plated in a 600-mm collagen I-coated dish (BD Biosciences, Bedford, MA) with 5 ml DMEM containing 10% FBS (Sigma-Aldrich, St. Louis, MO). After 5 days of incubation, fibroblasts migrated out of the tail pieces and were transferred to new dishes and allowed to proliferate.
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