Mouse anti flag epitope clone m2

Manufactured by Merck Group

The Mouse anti-FLAG epitope (clone M2) is a monoclonal antibody that specifically recognizes the FLAG epitope tag. The FLAG epitope is a short amino acid sequence (DYKDDDDK) that can be fused to recombinant proteins to facilitate detection and purification. The Mouse anti-FLAG epitope (clone M2) provides a reliable tool for the identification and isolation of FLAG-tagged proteins.

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Lab products found in correlation

Rabbit anti flag m2, by Merck Group (1 mentions) Rabbit anti ha, by Fortis Life Sciences (1 mentions) Mouse anti ha, by Merck Group (1 mentions) Anti flag m2 magnetic bead, by Merck Group (1 mentions) Mouse anti β actin clone ac 74, by Merck Group (1 mentions)

2 protocols using mouse anti flag epitope clone m2

Immunoblotting of Transfected Murine Cells

Cited 1 time

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12 well plates of transfected murine 8B9 cells were lysed in 0.5X IGEPAL lysis buffer as described previously [27 (link)]. All lysates were resolved by SDS-PAGE electrophoresis and transferred to PVDF membranes. Antibodies: rabbit anti-HA (Bethyl Laboratories, Inc.), mouse anti-HA (MAb clone 12CA5), rabbit anti-FLAG M2 (Sigma), mouse anti-p53 (MAb clone Ab-8, ThermoFisher Scientific), mouse anti-FLAG epitope (clone M2, Sigma) and anti-16E6 MAb 6G6 (a generous gift from Arbor Vita Corporation). Detection of blot signals was captured and analyzed using a Syngene G:Box (Syngene USA, Frederick, Maryland).

Drews C.M., Brimer N, & Vande Pol S.B. (2020). Multiple regions of E6AP (UBE3A) contribute to interaction with papillomavirus E6 proteins and the activation of ubiquitin ligase activity. PLoS Pathogens, 16(1), e1008295.

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Antibodies Used in Influenza Research

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Rabbit anti-GPS1 (clone 16-30), mouse anti-β-actin (clone AC-74), mouse anti-FLAG epitope (clone M2), and anti-FLAG M2 magnetic beads were purchased from Sigma-Aldrich (St. Louis, MO). Mouse anti-M2 antibody (antibody rM2ss23) (46 (link)), anti-PB2 antibody (antibody 18/1) (47 (link)), anti-PB1 antibody (antibody 136/1), and anti-PA antibody (antibody 55/2) (48 (link)) were kindly provided by Ayato Takada, Hokkaido University. The mouse anti-M1 antibody (antibody WS-27/52), mouse anti-HA antibody (antibody WS3-54), and mouse anti-Aichi NP antibody (antibody 2S-347/3) were produced in our laboratory at the University of Wisconsin.

Kuwahara T., Yamayoshi S., Noda T, & Kawaoka Y. (2019). G Protein Pathway Suppressor 1 Promotes Influenza Virus Polymerase Activity by Activating the NF-κB Signaling Pathway. mBio, 10(6), e02867-19.

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