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Galanthis nivalis lectin

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Galanthis Nivalis lectin is a protein isolated from the plant Galanthus nivalis, also known as the common snowdrop. It functions as a carbohydrate-binding protein, with the ability to recognize and interact with specific sugar molecules present on cell surfaces.

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Lab products found in correlation

Silver staining, by Thermo Fisher Scientific (2 mentions) Bn page, by Thermo Fisher Scientific (2 mentions) Amicon ultra 4 50k mwco centrifugation filter, by Merck Group (2 mentions) Sypro ruby protein staining, by Lonza (2 mentions)

2 protocols using galanthis nivalis lectin

1

Salipro-HIV-Spike Purification Protocol

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Salipro-HIV-spikes were purified using Galanthis Nivalis lectin (Sigma-Aldrich) affinity chromatography. The lectin binds to high mannose sugar moieties on the spike protein. The column was washed with 50 column volumes HN buffer followed by elution of the Salipro-HIV-spike particles using 0.3M methyl-αD-mannopyranoside. The methyl-αD-mannopyranoside was removed from the sample using a spin SEC column (Zeba Spin Desalting Columns, 7K MWCO) and changed to HN buffer. The Salipro-HIV-spikes were concentrated by ultra filtration using an Amicon Ultra-4 50K MWCO centrifugation filter (Millipore) and sample purity was assayed with SDS-PAGE using Sypro Ruby protein staining (Lonza group Ltd) and BN-PAGE using silver staining (Thermo Scientific).
Frauenfeld J., Löving R., Armache J.P., Sonnen A., Guettou F., Moberg P., Zhu L., Jegerschöld C., Flayhan A., Briggs J.A., Garoff H., Löw C., Cheng Y, & Nordlund P. (2016). A novel lipoprotein nanoparticle system for membrane proteins. Nature methods, 13(4), 345-351.
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2

Salipro-HIV-Spike Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Salipro-HIV-spikes were purified using Galanthis Nivalis lectin (Sigma-Aldrich) affinity chromatography. The lectin binds to high mannose sugar moieties on the spike protein. The column was washed with 50 column volumes HN buffer followed by elution of the Salipro-HIV-spike particles using 0.3M methyl-αD-mannopyranoside. The methyl-αD-mannopyranoside was removed from the sample using a spin SEC column (Zeba Spin Desalting Columns, 7K MWCO) and changed to HN buffer. The Salipro-HIV-spikes were concentrated by ultra filtration using an Amicon Ultra-4 50K MWCO centrifugation filter (Millipore) and sample purity was assayed with SDS-PAGE using Sypro Ruby protein staining (Lonza group Ltd) and BN-PAGE using silver staining (Thermo Scientific).
Frauenfeld J., Löving R., Armache J.P., Sonnen A., Guettou F., Moberg P., Zhu L., Jegerschöld C., Flayhan A., Briggs J.A., Garoff H., Löw C., Cheng Y, & Nordlund P. (2016). A novel lipoprotein nanoparticle system for membrane proteins. Nature methods, 13(4), 345-351.
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