Sas 9.0 statistical

In order to estimate the kinetic parameters of Cumulative Gas production (GP), all the results of GP were fitted using the NLIN Procedure of the SAS 9.0 statistical software. The formula used is as follows: a = b × (1 − e^−ct), where: a = volume (mL) of gas production per 0.2 g DM substrate at time t; b = asymptotic gas production (mL) of 0.2 g DM substrate; c = rate of gas production per hour. The test data were preliminarily sorted by Excel, and the one-way ANOVA was performed by SAS 9.0 statistical software. Duncan’s multiple comparison test was used to calculate the SEM and p Values. Additionally, the linear and quadratic curve trend analysis (Orthogonal polynomial contrast) was carried out. p < 0.05 (significant) and p < 0.01 (extremely significant) were used as the criteria to judge the significance of differences.

SAS9.0 statistical software was used to generate random numbers and analyze the data. The IBS—QOL and VAS measure were taken as the evaluation standard of the influence of QOL and abdominal pain before and after the education program. EpiData 3.1 was used to record the data in the IBS—QOL Scale and abdominal pain scores, which were evaluated by SPSS17.0 statistical analysis. Comparisons between categorical data were conducted using the Chi-square test, and the scoring data were evaluated by the student’s t test between education group and no-education group, and by the factorial design analysis of variance in different years between education group and no-education group, with P = 0.05 as the cutoff.