Accu chek performa blood glucose monitor

All rats were fasted for 8 h (07:00 am–03:00 pm) before evaluation. Both groups, diabetic and non-diabetic rats, were subdivided (n = 8 by subgroup) for the test of two doses of ZnONPs, 10 and 100 mg/kg body weight by two administration routes, oral or intraperitoneal. Before each administration, the ZnONPs dispersion was previously vortexed for 30 seconds to maintain its homogeneity.
Glycemia was evaluated at time 0 and 15, 30, 60, 90, 120, 240, and 360 min ZnONPs post-administration using an Accu-chek^® Performa blood glucose monitor (Roche Diagnostics, Mannnheim, Germany). The blood sample was obtained from the distal part of pre-cleaned rat tail using an alcohol swab; immediately after, a small cut was made with scissors and the blood obtained is deposited on the test strip and placed on the digital glucometer. The clot was removed for future fresh blood collection to perform the glucose measurement. This procedure is repeated with each rat.

After the BP measurement had been taken, blood glucose was determined using the Accu‐Chek Performa blood glucose monitor (Roche, Germany). Participants were asked when they had last eaten. If the participant reported not to have eaten within the last 8 h their glucose value was considered as Fasting Blood Glucose (FBG), all other values were considered Random Blood Glucose (RBG).
Thereafter, participants were asked to take off their shoes for measuring their height (Thermocare, height measurement scale) and weight (Omron weighing scale) to assess BMI.
Results were discussed with the participant and appropriate advice followed the obtained measurements (for example, participants were recommended to visit their doctor if the values obtained fell outside the normality range). Finally, a printed report was handed to the participants or sent by SMS if this was preferred (see Figure S2).