Tsa signal amplification kit

Manufactured by PerkinElmer

The TSA signal amplification kit is a laboratory equipment product designed to amplify signals in various analytical techniques. It functions by enhancing the detection and visualization of target analytes, enabling more sensitive and accurate measurements.

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Lab products found in correlation

Microm hm550 cryostat, by Thermo Fisher Scientific (3 mentions) Streptavidin biotin blocking kit, by Vector Laboratories (1 mentions) Tnb buffer, by PerkinElmer (1 mentions) Alexa fluor 488 conjugated anti rat igg, by Thermo Fisher Scientific (1 mentions) Biorevo fluorescence microscope, by Keyence (1 mentions) Vectashield, by Vector Laboratories (1 mentions) Streptavidin hrp, by PerkinElmer (1 mentions) Donkey anti mouse igg dylight594, by Jackson ImmunoResearch (1 mentions) Streptavidin alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Las x, by Leica (1 mentions) Keratin 5, by Fortrea (1 mentions)

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TSA kit (28 citations) TSA amplification kit (7 citations) TSA amplification (5 citations)

2 protocols using tsa signal amplification kit

Multicolor Immunofluorescent Staining of Mouse Spleen

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Spleen was embedded in OCT compound (Sakura Finetechnical) and frozen in liquid N₂. The tissue segments were sectioned with a cryostat at 8 μm. Frozen sections were fixed in cold acetone and blocked in TNB buffer (PerkinElmer Life Science) containing 5% normal rat serum. To block endogenous biotin, the sections were further treated with the Streptavidin/Biotin Blocking Kit (Vector Laboratories), and endogenous peroxidase activity was quenched with 1% H₂O₂. The primary Abs were anti-CD19-FITC mAb (cat#553785, 1D3; BD Biosciences), anti-mouse dendritic cell marker-biotin mAb (cat#130-101-843, 33D1; Miltenyi Biotec), anti-CD11c mAb-biotin mAb (cat#553800, HL3; BD Biosciences) and anti-CD3ɛ-APC mAb (cat#100312, 145-2C11; Biolegend). The CD19 signal was amplified by incubation with Alexa Fluor 488-conjugated anti-rat IgG (cat#A11006; Life Technologies). The 33D1 and CD11c staining was revealed with a TSA signal amplification kit (PerkinElmer Life Science) according to the manufacturer's instructions. The sections were incubated with Streptavidin-HRP (PerkinElmer Life Science) followed by Tyramide-Cy3 conjugate. At the end of the staining, slides were washed and mounted with Vectashield (Vector Laboratories). The stained slides were examined with a BIOREVO fluorescence microscope (BZ-9000; KEYENCE).

Uto T., Fukaya T., Takagi H., Arimura K., Nakamura T., Kojima N., Malissen B, & Sato K. (2016). Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity. Nature Communications, 7, 11273.

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Immunohistochemical Analysis of Ebi2-Deficient Thymus

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Ebi2^+/+ and Ebi2^-/- 1-month thymi were embedded in Tissue-Tek OCT (Sakura) and snap frozen. 6-9 μm cryosections were prepared on a Microm HM550 Cryostat (ThermoFisher), stored at -80°C, fixed in 100% acetone at -20°C for 15min and washed 2X in PBS + 0.1% Tween 20. Immunostaining was carried out for 1h at 4°C with the following primary antibodies: anti-Pancytokeratin-FITC (C-11; Sigma-Aldrich), -Keratin5 (rabbit polyclonal antibody; Covance), -CD4-APC (FM4-5; eBiosicence), -CD8-Alexa Fluor 488(53-6.7; eBioscience), CD11c-biotin (N418; BioLegend). The TSA Signal Amplification kit (Perkin Elmer) was used to detect anti-CD11c. Donkey anti-rabbit IgG DyLight 594 (Jackson ImmunoResearch Laboratory) and Streptavidin Alexafluor 647 (Life Technologies) secondary reagents were used for detection. Images were acquired on a DMi8 (Leica) microscope with 10x/0.4 and 20x/0.7 objectives, and processed uniformly using LasX (Leica) software.
To detect autoantibodies in mouse serum, 6-9 μm cryosections from Rag2^-/- kidney sections were prepared. Cryosections were fixed in acetone as described, and incubated overnight with undiluted mouse serum at 4°C. Donkey anti-mouse IgG DyLight594 (Jackson ImmunoResearch Laboratory) was used to detect murine auto-antibodies, followed by a DAPI nuclear counterstain.

Ki S., Thyagarajan H.M., Hu Z., Lancaster J.N, & Ehrlich L.I. (2017). EBI2 contributes to the induction of thymic central tolerance by promoting rapid motility of medullary thymocytes. European journal of immunology, 47(11), 1906-1917.

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