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Cy3 conjugated goat anti rat igg

Manufactured by Abcam
Sourced in United Kingdom, United States

CY3-conjugated goat anti-rat IgG is a secondary antibody that binds to rat immunoglobulin G (IgG) and is labeled with the fluorescent dye Cyanine 3 (Cy3). This antibody can be used for detection and visualization of rat IgG in various immunoassays and imaging applications.

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2 protocols using cy3 conjugated goat anti rat igg

1

Fibrosis-Inhibiting HSYA Protocol

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HSYA (C27H32O16; MW, 612.53; 98% Purity; Solarbio, Beijing, China), lecithin (Aladdin, Shanghai, China), bleomycin (BLM Biofeng, Shanghai, China), pirfenidone (Aladdin, Shanghai, China), ELISA kits (for TGF-β1 and IL-6; Cloud-Clone, Wuhan, China), 4% paraformaldehyde (Solarbio, Beijing, China), hematoxylin and eosin staining kit (beyotime, Shanghai, China), Weigert hematoxylin staining solution (Solarbio, Beijing, China), Ponceau S and Magenta staining solution (Solarbio, Beijing, China), phosphomolybdic acid solution (Solarbio, Beijing, China), Aniline blue solution (Solarbio, Beijing, China), DAPI (Solarbio, Beijing, China), RIPA buffer (Thermo Fisher Scientific, Waltham, USA), antibodies (for p38, p-p38, Smad3, collagen I, β-actin (Servicebio, Wuhan, China), anti-mouse IgG (Abcam, Cambridge, UK), anti-rabbit IgG (Abcam, Cambridge, UK)), BCA Protein Assay Reagent Kit (beyotime, Shanghai, China), and primary antibodies (CD45, α-SMA, and collagen I (Abcam, Cambridge, UK)), FITC-conjugated goat anti-rabbit IgG (Abcam, Cambridge, UK), CY3-conjugated goat anti-rat IgG (Abcam, Cambridge, UK), and CY5-conjugated goat anti-mouse IgG (Abcam, Cambridge, UK), were purchased.
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2

Evaluating Stem Cell Survival and Differentiation in Infarcts

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Immunofluorescence staining was performed to evaluate the survival, proliferation, and differentiation of EdU-labeled CDCs cells in the infarct area. The tissue section was immunostained for EdU using the Cell-Light™ EdU Apollo®567 Cell Tracking Kit according to the manufacturer’s protocol. Meanwhile, for immunostaining for α-sarcomeric actin, the tissue section was incubated with the primary antibody against α-sarcomeric actin (1:150, Abcam, USA), followed by Cy3-conjugated goat anti-rat IgG (1:100, Abcam, USA). Finally, 4’,6-diamidino-2-phenylindole (DAPI) (10236276001, Sigma) was used as a nuclear dye added in the immunofluorescence staining slide. A fluorescence was used to visualize the slide (BX51, OLYMPUS).
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