Azd 4547

NIH3T3 cells were plated at 10,000 cells per well in 96-well plates in sextuplets. Cells were treated for 72 h with various selective and non-selective FGFR inhibitors ranging from0.01 to 5000 nM followed by quantification of viable cells using an MTS/PMS colorimetric assay. FGFR inhibitors tested include BGJ398, JNJ-42756493, AZD-4547, Ponatinib, Dovitinib, and TAS-120 (Cayman Chemical). The half maximal inhibitory concentration (IC₅₀) of each FGFR inhibitor was calculated with GraphPad Prism using a four-parameter dose-response curve from four independent experiments.

NIH3T3 and 293T cells were plated at 10,000 cells per well and MMNK-1 were plated at 5,000 cells per well in a 96-well plate and allowed to adhere for 24 hours. Cells were dosed with varying concentrations (0.01 to 5000 nM) of FGFR inhibitors and allowed to incubate for 72 hours. FGFR inhibitors tested included infigratinib, AZD4547 (22 (link)), erdafitinib, TAS120 (23 (link)), dovitinib, and ponatinib (Cayman Chemical). Quantification of viability was carried out with the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay (Promega) per manufacturer’s protocol. The half maximal inhibitory concentration (IC₅₀) was calculated using GraphPad Prism. Four independent replicate experiments were conducted.