Organs bearing metastatic tumors were processed and the resulting cells collected and cultured. To isolate tumor cells from bone lesions, a 1 ml syringe with a 26G needle was filled with phosphate buffered saline (PBS) and inserted into one end of the tibia and cells expelled from the other end by applying positive pressure to the syringe. For adrenal and lung tumors, cells were crushed and homogenized using frosted glass slides and cells filtered to remove debris using a cell strainer (Falcon, USA). After one to two weeks of culture, a pure population of human cancer cells was obtained. Cell line protein expression was evaluated using with the Human Soluble Receptor Array Kit Non-Hematopoietic Panel or Human Oncology Array (R&D systems, Minneapolis, MN) according to the manufacturer’s instructions. Array results were quantified in terms of pixel density using ImageJ (NIH, Bethesda MD) and the degree of fold-change was determined in comparison to that of the parental line.
Human soluble receptor array kit non hematopoietic panel or human oncology array
Manufactured by R&D Systems
The Human Soluble Receptor Array Kit Non-Hematopoietic Panel or Human Oncology Array is a multiplex assay designed to detect and quantify the levels of various soluble human receptors in biological samples. The kit allows for the simultaneous measurement of multiple analytes in a single experiment, providing a comprehensive profile of the targeted receptors. This product is intended for research use only and the specific details of its intended use should be obtained from the manufacturer.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using human soluble receptor array kit non hematopoietic panel or human oncology array
1
Isolation and Characterization of Tumor Cells
2
Isolation and Characterization of Tumor Cells
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Organs bearing metastatic tumors were processed and the resulting cells collected and cultured. To isolate tumor cells from bone lesions, a 1 ml syringe with a 26G needle was filled with phosphate buffered saline (PBS) and inserted into one end of the tibia and cells expelled from the other end by applying positive pressure to the syringe. For adrenal and lung tumors, cells were crushed and homogenized using frosted glass slides and cells filtered to remove debris using a cell strainer (Falcon, USA). After one to two weeks of culture, a pure population of human cancer cells was obtained. Cell line protein expression was evaluated using with the Human Soluble Receptor Array Kit Non-Hematopoietic Panel or Human Oncology Array (R&D systems, Minneapolis, MN) according to the manufacturer’s instructions. Array results were quantified in terms of pixel density using ImageJ (NIH, Bethesda MD) and the degree of fold-change was determined in comparison to that of the parental line.
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