Skeletal muscle growth medium

Manufactured by Provitro

Skeletal muscle growth medium is a specialized cell culture medium designed to support the growth and maintenance of skeletal muscle cells in vitro. This medium provides the necessary nutrients, growth factors, and other components required for the proliferation and differentiation of skeletal muscle cells.

Automatically generated - may contain errors

Lab products found in correlation

Supplement mix, by Provitro (2 mentions) Dulbecco s modified eagle medium dmem, by Harvard Bioscience (1 mentions) Fetal calf serum (fcs), by Harvard Bioscience (1 mentions) Polyethylenimine (pei), by Merck Group (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Horse serum, by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Endothelial cell growth supplement (ecgs), by Corning (1 mentions) Penicillin, by Provitro (1 mentions) Streptomycin, by Provitro (1 mentions) Amphotericin, by Provitro (1 mentions) Streptomycin, by Harvard Bioscience (1 mentions) Dulbecco modified eagle medium (dmem), by Harvard Bioscience (1 mentions) Penicillin, by GE Healthcare (1 mentions) Streptomycin, by GE Healthcare (1 mentions) Penicillin, by Corning (1 mentions) Streptomycin, by Corning (1 mentions)

3 protocols using skeletal muscle growth medium

Cultivation and Transfection of Myoblast and HEK293T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

C2C12 mouse myoblasts and HEK293T cells were cultivated in Dulbecco’s modified Eagle Medium (DMEM; Biochrom AG) supplemented with 10% fetal calf serum (FCS; Biochrom AG), 2 mM L-glutamine and penicillin (100 units/ml)/streptomycin (10 µg/ml) (PAA) at 37°C and 10% or 5% CO₂, respectively. Immortalised human myoblasts were cultured in skeletal muscle growth medium (Provitro) supplemented with supplement mix (Provitro), 50 ng/ml amphotericin, 50 µg/ml gentamicin, 10% FCS, 2 mM L-glutamine and penicillin (100 units/ml)/streptomycin (10 µg/ml) at 37°C and 5% CO₂.
For transient transfection of HEK293T cells polyethylenimine (PEI; Sigma-Aldrich) was used. C2C12 cells were transfected using Lipofectamine2000 (Invitrogen) according to manufacturer’s instructions. For siRNA-mediated knockdown of human IRS4 Lipofectamine RNAiMAX (Invitrogen) was used according to manufacturer’s instructions; as control non-targeting siRNA was used (for detailed information see Supplementary Methods). In short, cells were transfected with 25 nM siRNA on two consecutive days and experiments were performed 72 h post-transfection.

Dörpholz G., Murgai A., Jatzlau J., Horbelt D., Belverdi M.P., Heroven C., Schreiber I., Wendel G., Ruschke K., Stricker S, & Knaus P. (2017). IRS4, a novel modulator of BMP/Smad and Akt signalling during early muscle differentiation. Scientific Reports, 7, 8778.

+ Open protocol

+ Expand

Myoblast Differentiation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Myoblasts (passage numbers between 3 and 6) were cultured in Skeletal Muscle Growth Medium (Provitro) supplemented with 10% foetal bovine serum (Provitro) and kept in an incubator at 37 °C with 5% CO₂. Differentiation was induced by treating cells with differentiation medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with GlutaMAX and 2% horse serum (all from Gibco^®) for 7 days. The medium was changed every 2 days.

Fontes-Oliveira C.C., Steinz M., Schneiderat P., Mulder H, & Durbeej M. (2017). Bioenergetic Impairment in Congenital Muscular Dystrophy Type 1A and Leigh Syndrome Muscle Cells. Scientific Reports, 7, 45272.

+ Open protocol

+ Expand

Culturing Diverse Cell Lines for Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293T, MCF7, and C2C12 cells were grown in DMEM (Biochrom AG) supplemented with 10% fetal calf serum (FCS; Biochrom AG), 2 mM l-glutamine, and penicillin (100 U/ml)/streptomycin (10 µg/ml) (PAA Laboratories) at 37°C and 10% (C2C12) or 5% CO₂. Immortalized human myoblasts were cultured in skeletal muscle growth medium (Provitro) supplemented with supplement mix (Provitro), 50 ng/ml amphotericin, 50 µg/ml gentamicin, 10% FCS, 2 mM l-glutamine, and penicillin (100 U/ml)/streptomycin (10 µg/ml) at 37°C and 5% CO₂. hFOBs (1.19) were cultured in a 1:1 mixture of DMEM and Ham’s F12 medium supplemented with 10% FCS, 2 mM l-glutamine, penicillin (100 U/ml)/streptomycin (10 µg/ml), and 0.3 mg/ml G418 (Biochrom AG) at 34°C with 5% CO₂ to keep them in a proliferative state. HUVECs were a kind gift from M. Lorenz and V. Stangl (Charité Universitätsmedizin, Berlin, Germany) and cultured on gelatin-coated tissue culture ware in M199 medium supplemented with 20% FCS, 50 µg/ml endothelial cell growth supplement (Corning), 25 µg/ml heparin, 2 mM l-glutamine, and penicillin (100 U/ml)/streptomycin (10 µg/ml) at 37°C and 5% CO₂. HUVECs were used at passage 3 in all experiments. Unless stated otherwise, all cells were starved for 5 h prior to stimulation with their respective growth medium, without FCS supplement, containing 2 mM l-glutamine and penicillin/streptomycin.

Brunner P., Hastar N., Kaehler C., Burdzinski W., Jatzlau J, & Knaus P. (2020). AMOT130 drives BMP-SMAD signaling at the apical membrane in polarized cells. Molecular Biology of the Cell, 31(2), 118-130.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!