Tae684

Manufactured by Santa Cruz Biotechnology

TAE684 is a tyrosine kinase inhibitor that targets the ALK (anaplastic lymphoma kinase) protein. It is a laboratory research tool used to study the effects of ALK inhibition in experimental models.

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Lab products found in correlation

Trametinib, by Santa Cruz Biotechnology (1 mentions) Gefitinib, by Santa Cruz Biotechnology (1 mentions) Doxorubicin, by Abcam (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Nutlin 3, by Selleck Chemicals (1 mentions) Rpmi medium, by Thermo Fisher Scientific (1 mentions) Ku 55933, by Santa Cruz Biotechnology (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Polyethylenimine (pei), by Polysciences (1 mentions) Amaxa nucleofector kit, by Lonza (1 mentions) Nucleofector 2 device, by Lonza (1 mentions) Sb203580, by Merck Group (1 mentions) Uo126, by Cell Signaling Technology (1 mentions) Sp600125, by Merck Group (1 mentions)

2 protocols using tae684

Cell Line Characterization and Transfection

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293T (human embryonic kidney), DLD-1, HCT-116 (colorectal carcinoma), MCF7, and BT474 (breast cancer) cells were obtained from ATCC; PC9 cells (NSCLC) were obtained from ECACC-Sigma-Aldrich; and Kelly cells (neuroblastoma) were a kind gift from Dr. C. Einvik. HCT-116 and MCF7 cells are WT for TP53 (http://p53.iarc.fr/CellLines.aspx). All cells were grown in DMEM (Life Technologies), except Kelly and PC9 cells, grown in RPMI medium (Life Technologies), both supplemented with 10% fetal bovine serum (Life Technologies) and 0.6% penicillin/streptomycin (Life Technologies). Cells were transfected with a Nucleofector II device (Lonza) using the Amaxa Nucleofector kit (Lonza) and electroporation program recommended by the manufacturer. 293T cells were transfected using polyethylenimine (Polysciences). The efficiency of each transfection was assessed in parallel using a GFP-containing plasmid.
Nutlin 3 was purchased from SelleckChem, and doxorubicin was purchased from Abcam. Gefitinib, WZ4002, TAE684, trametinib, and KU-55933 were purchased from Santa Cruz Biotechnology.

Guernet A., Mungamuri S.K., Cartier D., Sachidanandam R., Jayaprakash A., Adriouch S., Vezain M., Charbonnier F., Rohkin G., Coutant S., Yao S., Ainani H., Alexandre D., Tournier I., Boyer O., Aaronson S.A., Anouar Y, & Grumolato L. (2016). CRISPR-Barcoding for Intratumor Genetic Heterogeneity Modeling and Functional Analysis of Oncogenic Driver Mutations. Molecular cell, 63(3), 526-538.

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Inhibitors of Signaling Pathways

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UO126, an inhibitor of ERK kinase 1/2, was purchased from Cell Signaling Technology (Beverly, MA). SB203580 and SP600125, inhibitors of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase (JNK), respectively, were obtained from Sigma-Aldrich Corp. (St. Louis, MO) and Calbiochem (Bad Soden, Germany), respectively. IκB kinase-2 inhibitor IV was purchased from Santa Cruz Biotechnology (Dallas, TX). An inhibitor for HSP90, 17-allylamino-17-demethoxygeldanamycin, was purchased from Wako. TAE 684, an inhibitor for ALK, was obtained from Santa Cruz Biotechnology. These reagents were dissolved in dimethyl sulfoxide and used for experiments at the specified concentrations.

Watanabe M., Nakano K., Kadin M.E., Higashihara M., Watanabe T, & Horie R. (2017). CD30 Induces Heat Shock Protein 90 and Signal Integration in Classic Hodgkin Lymphoma Cells. The American journal of pathology, 187(1).

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