Hmgn2

Manufactured by Merck Group

HMGN2 is a high-mobility group nucleosome-binding protein that binds to nucleosomes and facilitates chromatin remodeling. It is a laboratory tool used for the study of chromatin structure and function.

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Lab products found in correlation

C ebpβ, by Santa Cruz Biotechnology (1 mentions) Acetyl α tubulin, by Cell Signaling Technology (1 mentions) Hdac6, by Abcam (1 mentions) Ac k2 hmgn2, by Cell Signaling Technology (1 mentions) Acetyl tubulin, by Cell Signaling Technology (1 mentions) Cyclin d1, by Santa Cruz Biotechnology (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Anti β tubulin, by Santa Cruz Biotechnology (1 mentions) Ecl reagent, by GE Healthcare (1 mentions)

2 protocols using hmgn2

Antibody Generation and Validation for Acetylation-Specific HMGN2

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Rabbit polyclonal ac-K2-HMGN2 antibody was made by New England Peptides. The antibody was raised against an acetylated N-terminal HMGN2 peptide (P(KAc)RKAEGDAC), followed by affinity purification. To ensure specificity, a blocking peptide (P(KAc)RKAEGDAC) and a non-acetyl blocking peptide (PKRKAEGDAC) were used to ensure acetyl-specific interaction. Antibodies used for western blotting; Tubulin (Invitrogen, 322500, 1:2000); HDAC6 (Abcam, ab47181, 1:500); HMGN2 (Millipore, 07-252, 1:500; Cell Signaling, #9437, 1:2000) pan-acetyl lysine (Millipore, AB3879, 1:500); CISH (Santa Cruz, sc-15344, 1:500); CyclinD1 (Santa Cruz, sc-718, 1:500); CEBPβ (Santa Cruz, sc-150, 1:500); PARP/Cleaved PARP (Cell Signaling, #9542, 1:500); ac-K2-HMGN2 (1:50); acetyl-α-Tubulin (Cell Signaling, #5335, 1:500). Secondary antibody was used at a dilution of 1:1000 for all antibodies except Tubulin, which was 1:2000. Antibodies used for IHC: CyclinD1 (Santa Cruz, sc-718, 1:1500); acetyl-Tubulin (Cell Signaling, D20G3, 1:100); ac-K2-HMGN2 (1:500).

Medler T.R., Craig J.M., Fiorillo A.A., Feeney Y.B., Harrell J.C, & Clevenger C.V. (2016). HDAC6 Deacetylates HMGN2 to Regulate Stat5a Activity and Breast Cancer Growth. Molecular cancer research : MCR, 14(10), 994-1008.

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Protein Analysis by Western Blot

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Cell lysates were analyzed on 12% SDS-PAGE gels. Following electrophoresis, the protein was transferred to polyvinylidene difluoride membrane (Millipore), immunoblotted, and detected with a horseradish peroxidase–conjugated secondary Ab and ECL reagents from GE Healthcare/Amersham Biosciences. The following polyclonal Abs were used to detect the proteins: anti-β-tubulin (1:1000 dilution; Santa Cruz Biotechnology), anti-Pitx2 (1:500 dilution; Capra Science), and Hmgn2 (1:500 dilution; Millipore).

Eliason S., Su D., Pinho F., Sun Z., Zhang Z., Li X., Sweat M., Venugopalan S.R., He B., Bustin M, & Amendt B.A. (2022). HMGN2 represses gene transcription via interaction with transcription factors Lef-1 and Pitx2 during amelogenesis. The Journal of Biological Chemistry, 298(9), 102295.

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