Net a

Manufactured by Merck Group

Sourced in United States

NET-A is a piece of laboratory equipment designed for the separation and detection of nucleic acids, such as DNA and RNA. It utilizes electrophoresis technology to separate molecules based on their size and charge. The core function of NET-A is to provide accurate and reliable analysis of nucleic acid samples.

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Lab products found in correlation

4 protocols using net a

Characterization of COS-1 Kidney Cells

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The COS-1 monkey kidney cell line was obtained from the ATCC and was
previously described [6 (link)]. Only
mycoplasma-negative cells were used in experiments. P₄, MPA, NET-A,
LNG, GES, ETG, NES, NoMAC, DRSP, Ald, spironolactone and phorbol
12-myristate13-acetate (PMA) were obtained from Sigma-Aldrich, RSA.
[³H]-Ald (83.4 Ci/mmol) was purchased from PerkinElmer Life and
Analytical Science, RSA.

Louw-du Toit R., Hapgood J.P, & Africander D. (2020). A direct comparison of the transcriptional activities of progestins used in contraception and menopausal hormone therapy via the mineralocorticoid receptor. Biochemical and biophysical research communications, 526(2), 466-471.

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Protein Expression Analysis in Cell Lines

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The following primary antibodies were obtained from Santa Cruz Biotechnology Inc., USA; GR(H-300): sc-8992, PR(C-20) (which detects PRA and B isoforms): sc-539, AR(441): sc-7305, GAPDH(0411): sc-47724, MR(MCR, H300): sc-11412, ERα(MC-20): sc-542. The flotillin-1 (610820) antibody was purchased from BD Transduction Laboratories (USA). The following secondary antibodies were obtained from Santa Cruz Biotechnology Inc., USA; anti-mouse: sc-2005, anti-goat: sc-2350 (used as IgG for the ChIP assay) and anti-rabbit: sc-2313. The ligands dexamethasone (DEX), MPA, P4, NET-A, NET, aldosterone (ALD) and mibolerone (MIB) were obtained from Sigma-Aldrich (South Africa). Human tumour necrosis factor α (TNFα) was obtained from Celtic Diagnostics (South Africa). Protease inhibitor cocktail tablets (EDTA-free) (cat #04693159001) were obtained from Roche (South Africa). Cycloheximide (CHX) was purchased from Sigma-Aldrich (South Africa).

Govender Y., Avenant C., Verhoog N.J., Ray R.M., Grantham N.J., Africander D, & Hapgood J.P. (2014). The Injectable-Only Contraceptive Medroxyprogesterone Acetate, Unlike Norethisterone Acetate and Progesterone, Regulates Inflammatory Genes in Endocervical Cells via the Glucocorticoid Receptor. PLoS ONE, 9(5), e96497.

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Receptor Binding Assay Protocol

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P₄, MPA, NET-A, levonorgestrel (LNG), gestodene (GES), nestorone (NES), nomegestrol acetate (NoMAC), drospirenone (DRSP), dihydrotestosterone (DHT), hydroxyflutamide (OHF), estradiol (E₂), fulvestrant (ICI 182,780) and phorbol 12-myristate13-acetate (PMA) were obtained from Sigma-Aldrich, RSA. Human tumor necrosis factor-alpha (TNFα) was obtained from Celtic Diagnostics, RSA. Unlabeled mibolerone (MIB) and [³H]-MIB (84.3 Ci/mmol) were purchased from PerkinElmer Life and Analytical Science, RSA, while [³H]-E₂ (100 Ci/mmol) was obtained from AEC-Amersham, RSA.

Louw-du Toit R., Perkins M.S., Hapgood J.P, & Africander D. (2017). Comparing the androgenic and estrogenic properties of progestins used in contraception and hormone therapy. Biochemical and biophysical research communications, 491(1), 140-146.

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Steroid Hormone Profiling Protocol

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MPA, NET-A, LNG, GES, NES, NoMAC, DRSP, pregnenolone (Preg), Prog, 17OH-Preg, 17OH-Prog, 16OH-Prog, deoxycorticosterone (DOC), corticosterone (CORT), 11-dehydrocorticosterone (11-DHC), aldosterone (Ald), deoxycortisol, cortisol, cortisone, dehydroepiandrosterone (DHEA), A4, testosterone, forskolin (FSK) and trilostane, were obtained from Sigma–Aldrich, South Africa, while 11β-hydroxyandrostenedione (11OH-A4) was purchased from Steraloids, USA. All test compounds, as well as FSK, were prepared in dimethylsulfoxide (DMSO), and added to the culturing medium at a final concentration of 0.2% DMSO. The deuterated internal standards, d2-testosterone, d9-Prog, d9-17OH-Prog and d4-cortisol were purchased from Cambridge Isotope Laboratories (Maryland, USA).

Louw-du Toit R., Perkins M.S., Snoep J.L., Storbeck K.H, & Africander D. (2016). Fourth-Generation Progestins Inhibit 3β-Hydroxysteroid Dehydrogenase Type 2 and Modulate the Biosynthesis of Endogenous Steroids. PLoS ONE, 11(10), e0164170.

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