Mouse tnf α polyclonal primary antibodies

The roots of Vladimiria souliei (Franch.) Ling were collected from Luding, Sichuan Province in September of 2015. This plant was identified by Professor Min Chen at Southwest University College of Pharmaceutical Sciences. A voucher specimen (No. CM2015-002) was deposited at the Southwest University College of Pharmaceutical Sciences (Chongqing, China).
LPS (Cat. No. L5293) was obtained from Sigma-Aldrich China, Co., LLC (Shanghai, China). D-GalN was purchased from Aladdin Reagent Database, Inc. (Shanghai, China). Diagnostic kits used for the determination of MDA, CAT, SOD, T-AOC. AST, ALT activities and cytochrome C (Cyto-C) assay kit were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Rabbit IL-6, IL-1β, IκB, p-IκB, IκB, p-IKK, NF-κB, Bax, Bcl-2, Cyto-C, Caspase3, Caspase8, Caspase9, β-actin, mouse TNF-α polyclonal primary antibodies, goat anti-mouse IgG-HRP-conjugated secondary antibody, and goat anti-rabbit IgGHRP-conjugated secondary antibody were obtained from Proteintech Group, Inc. (Wuhan, China), while total protein extraction kit was from Sangon Biotech, Co., Ltd. (Shanghai, China).

DIOS (purity 99% by HPLC) was provided by Chemistry Institute of Pharmaceutical Resource of Southwest University (China). LPS (Escherichia coli 055:B₅) and D-GalN were purchased from Sigma-Aldrich Co. LLC. (Shanghai, China). Diagnostic kits used for the determination of AST, ALT, CAT, iNOS, PGE₂, COX-2, MDA, SOD, and T-AOC activities were obtained from the Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). Rabbit IL-6, IL-1β, IκB, p-IκB, IKK, p-IKK, p38, p-p38, JNK, p-JNK, ERK, p-ERK, NF-κB (p65), Bax, Bcl-2, caspase 3, caspase 8, caspase 9, β-actin, mouse TNF-α polyclonal primary antibodies, goat anti-mouse IgG-HRP-conjugated secondary antibody and goat anti-rabbit IgG-HRP-conjugated secondary antibody were purchased from Proteintech (Wuhan, China), and total protein extraction kits were from Sangon Biotech Co. Ltd (Shanghai, China).